Rodgers J B, O'Brien R J, Balint J A
Am J Dig Dis. 1975 Mar;20(3):208-11. doi: 10.1007/BF01070723.
Labeled lecithin (32P, 2-3H-glycerol, 1-14C-palmitate) was prepared for studying absorption of lecithin by rat intestine and its incorporation into microsomal and prechylomicron-chylomicron lecithin. Bile fistula rats were perfused intraduodenally with bile salts and lecithin plus a liquid diet. Intestinal samples were obtained after sacrifice and specific activities (DPM/mumol) of isotopes in lecithin were determined. Ratios of specific activities of isotopes were calculated and compared to respective ratios in the original perfusate lecithin. Radioactivity rapidly appeared in jejunal lecithin following perfusion. When specific activities of isotopes in prechylomicron-chylomicron lecithin were compared to those in microsomal lecithin, specific activities were always greater in prechylomicron-chylomicron lecithin. Analysis of ratios of specific activities of isotopes in jejunal lecithin showed that the ratios were nearly identical to those in perfusate phospholipid, indicating that the lysolecithin portion of luminal lecithin can be absorbed intact and can then be utilized for jejunal lecithin synthesis.
制备了标记卵磷脂（32P、2-3H-甘油、1-14C-棕榈酸酯），用于研究大鼠肠道对卵磷脂的吸收及其掺入微粒体和前乳糜微粒-乳糜微粒卵磷脂的情况。对患有胆瘘的大鼠进行十二指肠内灌注胆汁盐、卵磷脂及流食。处死后获取肠道样本，并测定卵磷脂中同位素的比活度（每分钟衰变数/微摩尔）。计算同位素比活度的比率，并与原始灌注液卵磷脂中的相应比率进行比较。灌注后，放射性迅速出现在空肠卵磷脂中。当比较前乳糜微粒-乳糜微粒卵磷脂中同位素的比活度与微粒体卵磷脂中的比活度时，前乳糜微粒-乳糜微粒卵磷脂中的比活度总是更高。对空肠卵磷脂中同位素比活度比率的分析表明，这些比率与灌注液磷脂中的比率几乎相同，这表明肠腔卵磷脂的溶血卵磷脂部分可以完整吸收，然后可用于空肠卵磷脂的合成。
