Laloux L, Voisin M C, Allain J, Martin N, Kerboull L, Chevalier X, Claudepierre P
Department of Rheumatology, Hôpital Henri Mondor, 51 Avenue du Maréchal de Lattre de Tassigny, 94010 Créteil Cédex, France.
Ann Rheum Dis. 2001 Apr;60(4):316-21. doi: 10.1136/ard.60.4.316.
To determine which inflammatory cell types are present in entheses from patients with spondyloarthropathy (SpA) compared with patients with rheumatoid arthritis (RA) or osteoarthritis (OA).
Enthesis specimens were obtained during orthopaedic procedures in eight patients with SpA, four with RA, and three with OA. After decalcification, the lymphocyte subsets (CD3, CD4, CD8, CD20) in the bone marrow component of each enthesis were measured by an immunohistochemical technique.
Oedema and an inflammatory infiltrate were present in all the SpA specimens, being clearly predominant in the bone marrow component of the entheses. The density of all cell types in the bone marrow was significantly higher in the SpA group than in the two other groups. The cell type CD3+ showed the greatest difference between the SpA and RA groups, being increased fivefold in the SpA group. Within the SpA group, CD3+ cells were considerably more numerous than CD20+ cells-a difference from the RA group-and the predominant T cells were CD8+.
Persistent oedema with an inflammatory infiltrate composed predominantly of CD8+ cells was noted in the entheses of patients with SpA, being predominant in the bone marrow. These results suggest that CD8+ cells may have a key role in local inflammation in SpAs.
确定与类风湿关节炎（RA）或骨关节炎（OA）患者相比，脊柱关节炎（SpA）患者的起止点中存在哪些炎症细胞类型。
在骨科手术过程中获取8例SpA患者、4例RA患者和3例OA患者的起止点标本。脱钙后，采用免疫组织化学技术检测每个起止点骨髓成分中的淋巴细胞亚群（CD3、CD4、CD8、CD20）。
所有SpA标本均出现水肿和炎症浸润，在起止点的骨髓成分中明显占主导。SpA组骨髓中所有细胞类型的密度均显著高于其他两组。SpA组和RA组之间CD3+细胞类型差异最大，SpA组增加了五倍。在SpA组中，CD3+细胞比CD20+细胞多得多——这与RA组不同——且主要的T细胞是CD8+。
在SpA患者的起止点中发现持续水肿，并伴有主要由CD8+细胞组成的炎症浸润，且在骨髓中占主导。这些结果表明，CD8+细胞可能在SpA的局部炎症中起关键作用。
