高效的HIV-1整合需要聚（ADP-核糖）聚合酶-1。
Poly(ADP-ribose) polymerase-1 is required for efficient HIV-1 integration.
作者信息
Ha H C, Juluri K, Zhou Y, Leung S, Hermankova M, Snyder S H
机构信息
Department of Neuroscience, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
出版信息
Proc Natl Acad Sci U S A. 2001 Mar 13;98(6):3364-8. doi: 10.1073/pnas.051633498. Epub 2001 Mar 6.
Abstract
Poly(ADP-ribose) polymerase-1 (PARP-1; EC ) is an abundant nuclear enzyme, activated by DNA strand breaks to attach up to 200 ADP-ribose groups to nuclear proteins. As retroviral infection requires integrase-catalyzed DNA strand breaks, we examined infection of pseudotyped HIV type I in fibroblasts from mice with a targeted deletion of PARP-1. Viral infection is almost totally abolished in PARP-1 knockout fibroblasts. This protection from infection reflects prevention of viral integration into the host genome. These findings suggest a potential for PARP inhibitors in therapy of HIV type I infection.
摘要
聚（ADP-核糖）聚合酶-1（PARP-1；EC ）是一种丰富的核酶，被DNA链断裂激活后可将多达200个ADP-核糖基团连接到核蛋白上。由于逆转录病毒感染需要整合酶催化的DNA链断裂，我们检测了在PARP-1靶向缺失的小鼠成纤维细胞中I型假型HIV的感染情况。在PARP-1基因敲除的成纤维细胞中，病毒感染几乎完全被消除。这种对感染的保护作用反映了病毒整合到宿主基因组的过程被阻止。这些发现提示PARP抑制剂在治疗I型HIV感染方面具有潜力。
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本文引用的文献
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J Virol. 2000 Dec;74(23):11278-85. doi: 10.1128/jvi.74.23.11278-11285.2000.
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Poly(ADP-ribose) polymerase-1 in the nervous system.神经系统中的聚（ADP-核糖）聚合酶-1
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Evidence for regulation of NF-kappaB by poly(ADP-ribose) polymerase.聚（ADP - 核糖）聚合酶对核因子-κB调控的证据。
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Identification of poly(ADP-ribose) polymerase as a transcriptional coactivator of the human T-cell leukemia virus type 1 Tax protein.鉴定聚（ADP-核糖）聚合酶为人T细胞白血病病毒1型Tax蛋白的转录共激活因子。
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Inhibitors of strand transfer that prevent integration and inhibit HIV-1 replication in cells.链转移抑制剂可阻止整合并抑制HIV-1在细胞中的复制。
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