Smith G A, Gross S P, Enquist L W
Department of Molecular Biology, Schultz Building, Room 301, Princeton University, Princeton, NJ 08544-1014, USA.
Proc Natl Acad Sci U S A. 2001 Mar 13;98(6):3466-70. doi: 10.1073/pnas.061029798.
Alpha herpesviruses infect the vertebrate nervous system resulting in either mild recurrent lesions in mucosal epithelia or fatal encephalitis. Movement of virions within the nervous system is a critical factor in the outcome of infection; however, the dynamics of individual virion transport have never been assessed. Here we visualized and tracked individual viral capsids as they moved in axons away from infected neuronal cell bodies in culture. The observed movement was compatible with fast axonal flow mediated by multiple microtubule motors. Capsids accumulated at axon terminals, suggesting that spread from infected neurons required cell contact.
甲型疱疹病毒感染脊椎动物神经系统，可导致黏膜上皮出现轻度复发性损伤或致命性脑炎。病毒粒子在神经系统内的移动是感染结果的关键因素；然而，单个病毒粒子运输的动态过程从未得到评估。在这里，我们对单个病毒衣壳在培养物中从受感染的神经元细胞体向轴突移动的过程进行了可视化和追踪。观察到的移动与由多个微管马达介导的快速轴突运输相符。衣壳在轴突末端积累，这表明从受感染神经元传播需要细胞接触。
