Pearce M, Richter C P, Cheatham M A
Communication Sciences and Disorders, The Hugh Knowles Center, 2-240 Frances Searle Building, Northwestern University, Evanston, IL 60208-3550, USA.
J Neurosci Methods. 2001 Mar 30;106(1):57-67. doi: 10.1016/s0165-0270(01)00329-6.
Although it is traditional to perform sound calibrations in anesthetized animals by placing a probe-tube microphone near the tympanic membrane, these measurements are inaccurate at high stimulus frequencies where hearing must be quantified in the mouse. Hence, our motivation to develop another approach using the mouse ear canal as a coupler. Results of real-ear-canal calibrations indicate that an average calibration can be used to estimate sound pressure levels in the three mouse strains tested. Similar estimates were also obtained using a tubing coupler, whose volume was comparable to that of the ear canal, thereby offering a simpler alternative. In addition, real-head calibrations were also performed to provide a procedure that can be used in situations where the ear is not dissected, as in measurements of the auditory brainstem response. Calibrations for open, rather than closed, sound-delivery systems were also evaluated using a modified method of substitution.
虽然传统上是通过在鼓膜附近放置探针式麦克风对麻醉动物进行声音校准，但在必须对小鼠听力进行量化的高刺激频率下，这些测量是不准确的。因此，我们有动力开发另一种方法，即使用小鼠耳道作为耦合器。耳道实际校准结果表明，平均校准可用于估计所测试的三种小鼠品系中的声压水平。使用体积与耳道相当的管道耦合器也获得了类似的估计结果，从而提供了一种更简单的替代方法。此外，还进行了真实头部校准，以提供一种可用于未解剖耳朵的情况（如在听觉脑干反应测量中）的程序。还使用改进的替代方法评估了开放而非封闭的声音传输系统的校准。
