Senior J, Shah A, Monteux C, De Biasi V
SmithKline Beecham Pharmaceuticals R&D, New Frontiers Science Park, Third Avenue, Harlow, Essex CM19 5AW, UK.
J Pharm Biomed Anal. 2001 Mar;24(5-6):843-8. doi: 10.1016/s0731-7085(00)00551-3.
The use of Interdigitated Array (IDA) Microelectrodes for detection of low levels of biogenic amines has been demonstrated in stationary solutions and flow systems [M. Morita, et al., Electrochemica Acta 42 (20--21) (1997) 3177--3183]. This technique is highly sensitive. We have evaluated this technology as applied to High Pressure Liquid Chromatography with Electrochemical Detection (HPLC-EC) for analysis of microdialysate and tissue samples. With this new technology we demonstrated a x 10 fold increase in sensitivity in comparison to our existing technology. We are now able to detect dopamine at a level of 53 x 10(-18) moles on column and serotonin at 26 x 10(-18) moles on column. This technology now permits analysis of biogenic amines in samples from brain areas not previously amenable to this type of experiment.
叉指阵列（IDA）微电极用于检测低水平生物胺已在静态溶液和流动系统中得到证实[森田实等人，《电化学学报》42 (20 - 21) (1997) 3177 - 3183]。该技术高度灵敏。我们已评估此技术应用于高压液相色谱 - 电化学检测（HPLC - EC）以分析微透析液和组织样品的情况。通过这项新技术，我们证明与现有技术相比灵敏度提高了10倍。我们现在能够在柱上检测到53×10⁻¹⁸摩尔的多巴胺和26×10⁻¹⁸摩尔的血清素。这项技术现在允许对以前无法进行此类实验的脑区样品中的生物胺进行分析。
