Zgórka G, Kawka S
Department of Pharmacognosy, Medical University, 12 Peowiaków Street, 20-007 Lublin, Poland.
J Pharm Biomed Anal. 2001 Mar;24(5-6):1065-72. doi: 10.1016/s0731-7085(00)00541-0.
Free phenolic acids (PhAs) contained in methanolic extracts of Eleutherococcus senticosus roots and pharmaceutical preparations, deriving from this plant, were isolated by solid-phase extraction (SPE) and identified by reversed-phase high-performance liquid chromatography (RP-HPLC). To obtain precise, accurate and validated results of qualitative and quantitative analysis, ultraviolet (at lambda=254 nm), photodiode array (at lambda=254 and 280 nm) and fluorescence (at lambda(Ex)=230 or 265 nm and lambda(Em)=350 nm) detection was used. Additionally, the HPLC separation of PhAs on two different octadecyl sorbents: Hypersil (Shandon, UK) and Symmetry (Waters, USA) was performed. Eight PhAs: chlorogenic, protocatechuic, p-hydroxybenzoic, caffeic, vanillic, syringic, p-coumaric and ferulic, in different quantitative proportions, were identified, both in the roots of E. senticosus and pharmaceutical formulations examined.
通过固相萃取（SPE）分离刺五加根甲醇提取物及由此植物制成的药物制剂中所含的游离酚酸（PhAs），并采用反相高效液相色谱（RP-HPLC）进行鉴定。为获得定性和定量分析的精确、准确且经过验证的结果，使用了紫外检测（波长λ = 254 nm）、光电二极管阵列检测（波长λ = 254和280 nm）以及荧光检测（激发波长λ(Ex)=230或265 nm，发射波长λ(Em)=350 nm）。此外，还在两种不同的十八烷基吸附剂上进行了酚酸的HPLC分离：Hypersil（英国山顿）和Symmetry（美国沃特世）。在所检测的刺五加根和药物制剂中均鉴定出了8种酚酸，分别为绿原酸、原儿茶酸、对羟基苯甲酸、咖啡酸、香草酸、丁香酸、对香豆酸和阿魏酸，其含量比例各不相同。
