肺炎克雷伯菌O3脂多糖核心区域的结构。核心外部的3-脱氧-α-D-甘露糖辛酸（α-Kdo）残基，是肺炎克雷伯菌O1、O2、O3、O4、O5、O8和O12脂多糖的常见结构元件。
The structure of the core region of the lipopolysaccharide from Klebsiella pneumoniae O3. 3-deoxy-alpha-D-manno-octulosonic acid (alpha-Kdo) residue in the outer part of the core, a common structural element of Klebsiella pneumoniae O1, O2, O3, O4, O5, O8, and O12 lipopolysaccharides.
作者信息
Vinogradov E, Cedzynski M, Ziolkowski A, Swierzko A
机构信息
Institute for Biological Sciences, National Research Council, Ottawa, ON, Canada; Microbiology and Virology Centre, Polish Academy of Science, Lodowa, Lodz, Poland.
出版信息
Eur J Biochem. 2001 Mar;268(6):1722-9. doi: 10.1046/j.1432-1033.2001.02047.x.
The structure of lipid A-core region of the lipopolysaccharide (LPS) from Klebsiella pneumoniae serotype O3 was determined using NMR, MS and chemical analysis of the oligosaccharides, obtained by mild acid hydrolysis, alkaline deacylation, and deamination of the LPS: [carbohydrate structure see text] where P is H or alpha-Hep; J is H or beta-GalA; R is H or P (in the deacylated oligosaccharides). Screening of the LPS from K. pneumoniae O1, O2, O4, O5, O8, and O12 using deamination showed that they also contain alpha-Hep-(1-->4)-alpha-Kdo-(2-->6)-GlcN and alpha-Kdo-(2-->6)-GlcN fragments.
利用核磁共振（NMR）、质谱（MS）以及对通过温和酸水解、碱脱酰基和脂多糖（LPS）脱氨作用获得的寡糖进行化学分析，确定了肺炎克雷伯菌O3血清型脂多糖（LPS）的脂质A核心区域结构：[碳水化合物结构见正文]，其中P为H或α-庚糖；J为H或β-半乳糖醛酸；R为H或P（在脱酰基寡糖中）。使用脱氨作用对肺炎克雷伯菌O1、O2、O4、O5、O8和O12的LPS进行筛选表明，它们还含有α-庚糖-（1→4）-α-酮脱氧辛糖酸-（2→6）-葡糖胺和α-酮脱氧辛糖酸-（2→6）-葡糖胺片段。
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