A Novel Peptide as a Specific and Selective Probe for Detection.

is infamous for generating hospital-acquired infections, many of which are difficult to treat due to the bacterium's multidrug resistance. A sensitive and robust detection method of can help prevent a disease outbreak. Herein, we used cells as bait to screen a commercially available phage-displayed random peptide library for peptides that could be used to detect . The biopanning-derived peptide TSATKFMMNLSP, named KP peptide, displayed a high selectivity for the with low cross-reactivity to related Gram-negative bacteria. The specific interaction between KP peptide and lipopolysaccharide resulted in the peptide's selectivity against . Quantitative analysis of this interaction by enzyme-linked immunosorbent assay revealed that the KP peptide possessed higher specificity and sensitivity toward than commercially available anti- spp. antibodies and could detect at a detection limit of 10 CFU/mL. These results suggest that KP peptide can be a promising alternative to antibodies in developing a biosensor system for detection.