用于检测厌氧菌血症的血培养常规处理中的气液色谱法。
Gas-liquid chromatography in routine processing of blood cultures for detecting anaerobic bacteraemia.
作者信息
Reig M, Molina D, Loza E, Ledesma M A, Meseguer M A
出版信息
J Clin Pathol. 1981 Feb;34(2):189-93. doi: 10.1136/jcp.34.2.189.
Abstract
Gas-liquid chromatography was performed on 233 positive blood cultures and findings were compared with culture results. Obligate anaerobic bacteria were recovered from 78 out of 79 blood cultures containing butyric or iso-valeric acids, or both; from 28 out of 69 blood cultures containing succinic acid; and from only one out of 41 blood cultures containing succinic but not butyric or iso-valeric acid. Good correlations (88%) were found for the recovery of anaerobic bacteria and the detection of butyric and/or iso-valeric acids. Detecting volatile fatty acids by gas-liquid chromatography performed on blood cultures at the first signs of growth can therefore provide an early and reliable indication of the presence of anaerobic bacteria.
摘要
对233份阳性血培养标本进行了气液色谱分析，并将结果与培养结果进行了比较。在79份含有丁酸或异戊酸或两者皆有的血培养标本中，有78份培养出专性厌氧菌；在69份含有琥珀酸的血培养标本中，有28份培养出专性厌氧菌；而在41份仅含有琥珀酸而不含有丁酸或异戊酸的血培养标本中，只有1份培养出专性厌氧菌。发现厌氧菌的培养与丁酸和/或异戊酸的检测之间具有良好的相关性（88%）。因此，在血培养出现生长迹象的最初阶段通过气液色谱法检测挥发性脂肪酸，可以为厌氧菌的存在提供早期且可靠的指示。
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