Shanmugasundaram K R, Kumar S S, Rajajee S
Department of Medical Biochemistry, Dr. A.L.M.P.G. Institute of Basic Medical Sciences, University of Madras, Taramani Campus, - 600 113, Chennai, India.
Clin Chim Acta. 2001 Mar;305(1-2):107-14. doi: 10.1016/s0009-8981(00)00425-3.
The aim of the present study is to evaluate the biochemical parameters in blood relevant to oxygen free radicals and antioxidant defenses in children with asthma. A total of 210 asthmatic children, aged 5-18 years, were studied at two different times, once during a severe episode of wheeze (during episode category) and the other after recovery (resting condition). A total of 180 healthy children participated in the study as age and sex matched healthy controls. Superoxide and hydroxyl radical assays were used as a measure of free radical formation. Antioxidant enzymes and free radical scavengers in blood were also assayed. Lipid peroxidation products were assayed in plasma and erythrocytes to evaluate the imbalance (if any) between oxidant (radical) formation and their inactivation. Serum IgE concentrations and peak expiratory flow rate (PEFR) were used as measures of allergic reactions and residual lung capacity, respectively. Excessive production of superoxide and hydroxyl radicals were noted in the blood cells in asthmatics and were correlated to the severity of disease measured as PEFR. Superoxide dismutase and free radical scavengers in blood were significantly lower in asthma, even during resting condition. The present observations endorse the correlation between disease severity and oxygen radical production in asthma subjects. Oxygen metabolites may play a direct or indirect role in the modulation of airway inflammation. Excessive superoxide and hydroxyl radical production may be used as a marker for susceptibility to asthma and for monitoring therapeutic measures.
本研究的目的是评估哮喘患儿与氧自由基及抗氧化防御相关的血液生化参数。共有210名5至18岁的哮喘患儿在两个不同时间接受研究，一次是在严重喘息发作期间（发作期），另一次是在恢复后（静息状态）。共有180名健康儿童作为年龄和性别匹配的健康对照参与了该研究。超氧化物和羟基自由基测定被用作自由基形成的指标。还对血液中的抗氧化酶和自由基清除剂进行了测定。在血浆和红细胞中测定脂质过氧化产物，以评估氧化剂（自由基）形成与其失活之间的失衡（如有）。血清IgE浓度和呼气峰值流速（PEFR）分别用作过敏反应和残余肺容量的指标。哮喘患者血细胞中超氧化物和羟基自由基的产生过多，且与以PEFR衡量的疾病严重程度相关。即使在静息状态下，哮喘患者血液中的超氧化物歧化酶和自由基清除剂也显著降低。本观察结果证实了哮喘患者疾病严重程度与氧自由基产生之间的相关性。氧代谢产物可能在气道炎症的调节中起直接或间接作用。超氧化物和羟基自由基的过度产生可作为哮喘易感性和监测治疗措施的标志物。
