Wang Z P, Gu Z P, Cao L, Xu Y, You G D, Mao B Y, Qian S Z
Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 200031, China.
Asian J Androl. 1999 Sep;1(3):121-5.
To further evaluate the antifertility effects of tripchlorolide, a derivative of triptolide produced at the extraction procedure of Tripterygium wilfordii Hook. f., in male rats and to investigate its sites and possible mechanisms of action.
In male rats, tripchlorolide was given by oral garage at a dose of 50 micrograms.kg-1.d-1 for 5 weeks, fertility was assessed by mating tests, and biochemical indices and light microscopic observation of the epididymides and testes were also performed.
Administration of tripchlorolide at 50 micrograms.kg-1.d-1 for 3 weeks did not influence the fertility of male rats, but 5-week treatment rendered the rats infertile. The density and motility of spermatozoa collected from cauda epididymides were reduced significantly. The epididymal weights, as well as the L-carnitine concentration and alpha-glucosidase content in the epididymal fluid were decreased. There were no significant differences in alpha-glucosidase and acid phosphatase (ACP) in caput epididymal homogenates between the control and the experimental rats. Obvious morphological changes were observed in the epididymal spermatozoa, mainly including head and tail separation or acrosome curving. Sloughed spermatids were found in the seminifeous and epididymal tubules. In testicular homogenates, tripchlorolide had no influence on the lactate dehydrogenase-C4 (LDH-C4) and hyaluronidase activities. No apparent lesions were observed in the seminiferous and epididymal epithelium.
At the dose level employed, tripchlorolide has a significant effect on the fertility in male rats and the primary sites of action may be spermatids and testicular and epididymal spermatozoa.
进一步评估雷公藤多苷在提取过程中产生的雷公藤内酯醇衍生物雷公藤氯内酯醇对雄性大鼠的抗生育作用，并探讨其作用部位及可能的作用机制。
对雄性大鼠，以50微克·千克⁻¹·天⁻¹的剂量口服给予雷公藤氯内酯醇，持续5周，通过交配试验评估生育能力，并进行生化指标检测以及附睾和睾丸的光镜观察。
以50微克·千克⁻¹·天⁻¹的剂量给予雷公藤氯内酯醇3周对雄性大鼠的生育能力没有影响，但5周的治疗使大鼠不育。从附睾尾部收集的精子密度和活力显著降低。附睾重量以及附睾液中的左旋肉碱浓度和α-葡萄糖苷酶含量均降低。对照大鼠和实验大鼠附睾头部匀浆中的α-葡萄糖苷酶和酸性磷酸酶（ACP）没有显著差异。在附睾精子中观察到明显的形态学变化，主要包括头部和尾部分离或顶体弯曲。在生精小管和附睾小管中发现了脱落的精子细胞。在睾丸匀浆中，雷公藤氯内酯醇对乳酸脱氢酶-C4（LDH-C4）和透明质酸酶活性没有影响。在生精上皮和附睾上皮中未观察到明显病变。
在所采用的剂量水平下，雷公藤氯内酯醇对雄性大鼠的生育能力有显著影响，其主要作用部位可能是精子细胞以及睾丸和附睾中的精子。
