Shang Xue-Jun, Liu Qing-Zhen, Kang Ning, Huang Yu-Feng
Department of Andrology, Nanjing General Hospital of Nanjing Military Region, Nanjing, Jiangsu 210002, China.
Zhonghua Nan Ke Xue. 2009 Dec;15(12):1090-4.
To investigate the protective effect of L-carnitine on the testis and epididymis against ornidazole (ORN)-induced injury in male rats.
Forty male SD rats weighing 200 -230 g were randomly divided into 5 groups, Group A treated with 0.5% sodium carboxymethyl cellulose, and Groups B, C, D and E with ORN at the daily dose of 400 mg/kg, 800 mg/kg, 400 mg/kg plus LC 100 mg/kg and 800 mg/kg plus LC 100 mg/kg, respectively, all by oral gavage for 20 days continuously. Twenty-four hours after the last administration, all the rats were put to death, their testes and epididymides harvested, weighed and subjected to HE staining. The indexes of the testes and epididymides were obtained and their histopathological changes observed.
Compared with Group A, Groups B and C showed significant decreases in the indexes of the testis and epididymis (P < 0.05 and P < 0.01), while Group D exhibited no difference and Group E extremely significant difference (P < 0.01). HE staining revealed that the spermatogenic cells at all levels of testicular seminiferous tubules were neatly arranged in Group B, caduceus in some seminiferous tubules, with decreased number of sperm and sporadic spermatogenic cells in the epididymal duct. Necrotic and caduceus spermatogenic cells were observed in the seminiferous tubules of Group C, with significantly decreased number of sperm and lots of non-sperm cell components in the epididymal duct. No obvious changes were found in the testicular seminiferous tubules, nor evident reduction in the number of sperm in the epididymal duct of Group D. Group E showed decreased number of sperm in the testicular seminiferous tubules, necrotic and caduceus spermatogenic cells, obviously reduced number of sperm and a lot of non-sperm cell components in the epididymal duct.
ORC can induce histopathological changes in the testis and epididymis of male rats, and L-carnitine plays a role in protecting the testis and epididymis from ORN-induced injury in male rats.
探讨左旋肉碱对奥硝唑（ORN）诱导的雄性大鼠睾丸和附睾损伤的保护作用。
将40只体重200 - 230 g的雄性SD大鼠随机分为5组，A组给予0.5%羧甲基纤维素钠，B、C、D、E组分别给予每日剂量为400 mg/kg、800 mg/kg、400 mg/kg加LC 100 mg/kg、800 mg/kg加LC 100 mg/kg的ORN，均通过灌胃连续给药20天。末次给药24小时后，处死所有大鼠，摘取睾丸和附睾，称重并进行HE染色。获取睾丸和附睾的指标，观察其组织病理学变化。
与A组相比，B组和C组睾丸和附睾指标显著降低（P < 0.05和P < 0.01），而D组无差异，E组差异极显著（P < 0.01）。HE染色显示，B组睾丸生精小管各级生精细胞排列整齐，部分生精小管有细胞脱落，附睾管内精子数量减少，生精细胞散在分布。C组生精小管可见坏死和脱落的生精细胞，附睾管内精子数量显著减少，有大量非精子细胞成分。D组睾丸生精小管无明显变化，附睾管内精子数量无明显减少。E组睾丸生精小管内精子数量减少，有坏死和脱落的生精细胞，附睾管内精子数量明显减少，有大量非精子细胞成分。
ORN可诱导雄性大鼠睾丸和附睾组织病理学改变，左旋肉碱对ORN诱导的雄性大鼠睾丸和附睾损伤具有保护作用。
