Premakumara G A, Ratnasooriya W D, Tillekeratne L M, Amarasekare A S
Natural Products Development Group, Industrial Technology Institute (CISIR), Colombo, Sri Lanka.
Asian J Androl. 2001 Mar;3(1):27-31.
To evaluate the sperm motility stimulating activity of a sulfono glycolipid (S-ACT-1) isolated from Gelidiella acerosa, a Sri Lankan marine red algae.
S-ACT-1, a white amorphous powder was separated from more polar fractions of the hexane soluble of 1:1 CH2Cl2/MeOH extract and subjected to 1H, 13C NMR and IR Spectroscopy after reverse phase HPLC for identification. Effects of S-ACT-1 on human sperm motility was assessed in vitro at 10, 100 and 1,000 microg/mL concentrations at 37 degrees C for 0, 5, 15, 30 and 60 min.
S-ACT-1 was identified as a glycolipid sulfate. The lower dose increased the sperm motility slightly, whilst the medium dose significantly increased the motility (P < 0.05) from 5 min of incubation reaching a peak at 15 min and the stimulant effect was sustained throughout the experimental period. Furthermore, the medium dose rendered 80% of the immotile viable sperm motile. In contrast, the highest dose impaired the sperm motility. The sperm stimulating activity of S-ACT-1 was dose-dependent and had a bell-shaped dose response curve for all the 5 incubation periods.
S-ACT-1 of Gelidiella acerosa is a Sulfono glycolipid. S-ACT-1 has a potent sperm motility stimulating activity in vitro and has the potential to be developed into a sperm stimulant.
评估从斯里兰卡海洋红藻粗江蓠中分离出的一种磺基糖脂（S-ACT-1）对精子活力的刺激活性。
S-ACT-1为白色无定形粉末，从二氯甲烷/甲醇（1:1）提取物的己烷可溶部分的极性更大的馏分中分离出来，经反相高效液相色谱法分离后，进行¹H、¹³C核磁共振和红外光谱鉴定。在37℃下，分别于0、5、15、30和60分钟时，评估S-ACT-1在10、100和1000μg/mL浓度下对人类精子活力的体外影响。
S-ACT-1被鉴定为一种硫酸糖脂。较低剂量略微增加了精子活力，而中等剂量在孵育5分钟时显著增加了精子活力（P<0.05），在15分钟时达到峰值，并且在整个实验期间刺激作用持续存在。此外，中等剂量使80%的无活力活精子具有活力。相比之下，最高剂量损害了精子活力。S-ACT-1的精子刺激活性呈剂量依赖性，在所有5个孵育期均呈现钟形剂量反应曲线。
粗江蓠中的S-ACT-1是一种磺基糖脂。S-ACT-1在体外具有强大的精子活力刺激活性，有潜力开发成为一种精子兴奋剂。
