Premakumara G A, Ratnasooriya W D, Tillekeratne L M
Department of Zoology, University of Colombo, Sri Lanka.
Contraception. 1996 Dec;54(6):379-83. doi: 10.1016/s0010-7824(96)00198-9.
This study reports the isolation of a non-steroidal contragestative agent from the Sri Lankan marine red alga, Gelidiella acerosa. One-hundred grams of MeOH-CH2Cl2 (1:1) crude extract of G. acerosa was initially partitioned into hexane, CH2Cl2 and EtOAc, respectively, using the modified Kupchan scheme. At each separation step, all the resulting fractions were tested for contragestative effects in pregnant rats. The fertility was assessed in terms of the number of viable implants following oral administration of fractions (co-precipitated with polyvinylpyrrolidone), on day 1 through day 7 of pregnancy. The hexane fraction with highest activity was subjected to gel filtration through Sephadex LH-20, vacuum liquid chromatography over TLC-grade silica, two open-column chromatography runs over silica gel (60-120 mesh), and HPLC to isolate the active component. Nuclear magnetic resonance and infrared spectroscopic analysis revealed that the contragestative agent of G. acerosa is a sphingosine derivative.
本研究报告了从斯里兰卡海洋红藻Gelidiella acerosa中分离出一种非甾体类抗孕剂。首先，采用改良的库普昌方法，将100克G. acerosa的甲醇 - 二氯甲烷（1:1）粗提物分别用己烷、二氯甲烷和乙酸乙酯进行分配。在每个分离步骤中，对所有得到的馏分进行妊娠大鼠抗孕作用测试。在妊娠第1天至第7天，通过口服各馏分（与聚乙烯吡咯烷酮共沉淀）后，根据存活植入物的数量评估生育力。将活性最高的己烷馏分通过Sephadex LH - 20进行凝胶过滤、在TLC级硅胶上进行真空液相色谱、在硅胶（60 - 120目）上进行两次开放柱色谱以及高效液相色谱，以分离活性成分。核磁共振和红外光谱分析表明，G. acerosa的抗孕剂是一种鞘氨醇衍生物。
