乳腺癌及癌旁乳腺组织中MUC5AC和MUC6表达的免疫组织化学研究
Immunohistochemical study of the expression of MUC5AC and MUC6 in breast carcinomas and adjacent breast tissues.
作者信息
Pereira M B, Dias A J, Reis C A, Schmitt F C
机构信息
Instituto de Patologia e Imunologia Molecular da Universidade do Porto, Portugal.
出版信息
J Clin Pathol. 2001 Mar;54(3):210-3. doi: 10.1136/jcp.54.3.210.
AIM
To study the protein expression patterns of MUC5AC and MUC6 in normal and diseased breast tissues and to compare their expression with that of a mucin (MUC1) normally expressed in mammary tissues.
METHODS
Formalin fixed, paraffin wax embedded tissue from 69 cases of invasive breast carcinoma and surrounding breast tissue was studied immunohistochemically with monoclonal antibodies against MUC1 (SM3), MUCSAC (CLH2), and MUC6 (CLH6), using the avidin-biotin-peroxidase method.
RESULTS
MUC5AC was detected in five of 68 cases of invasive carcinoma including one of three cases of pure colloid carcinoma. MUCSAC expression in the adjacent normal breast epithelium was present in one of 29 cases and in one of two cases of ductal carcinoma in situ. None of 15 cases of ductal hyperplasia without atypia was positive for MUCSAC. MUC6 was present in 15 of 65 cases of invasive carcinoma, in four of 29 cases of normal adjacent epithelium, two of 15 cases of ductal hyperplasia without atypia, and one of two cases of ductal carcinoma in situ. MUC1 immunoreactivity detected by the SM3 antibody was present in 50 of the 67 cases of invasive carcinoma, but expression was also detected in benign epithelium. All invasive carcinomas expressing MUCSAC were positive for MUC1 and four were positive for MUC6. No significant association was found between the expression of these mucins and tumour size, histological grade, node status, oestrogen receptor status, p53 positivity, or c-ErbB-2 overexpression.
CONCLUSIONS
This study documents the expression of two different mucins (MUCSAC and MUC6) not described as being expressed by normal breast tissues in a minority of breast carcinomas, as well as in normal and hyperplastic epithelium. Although the role of mucins in malignant transformation and the progression of breast cancer is not well understood, in some cases, there is probably an upregulation of several genes that encode distinct mucin proteins.
目的
研究黏蛋白5AC（MUC5AC）和黏蛋白6（MUC6）在正常及病变乳腺组织中的蛋白表达模式，并将它们的表达与乳腺组织中正常表达的一种黏蛋白（MUC1）的表达进行比较。
方法
采用抗生物素蛋白-生物素-过氧化物酶法，用抗MUC1（SM3）、MUC5AC（CLH2）和MUC6（CLH6）的单克隆抗体，对69例浸润性乳腺癌及周围乳腺组织的福尔马林固定、石蜡包埋组织进行免疫组织化学研究。
结果
在68例浸润性癌中的5例检测到MUC5AC，其中包括3例纯胶样癌中的1例。在29例相邻正常乳腺上皮中，MUC5AC表达见于1例，在2例导管原位癌中的1例中表达。15例无异型性的导管增生中无1例MUC5AC呈阳性。65例浸润性癌中的15例、29例相邻正常上皮中的4例、15例无异型性的导管增生中的2例以及2例导管原位癌中的1例中有MUC6表达。SM3抗体检测到的MUC1免疫反应性见于67例浸润性癌中的50例，但在良性上皮中也检测到表达。所有表达MUC5AC的浸润性癌MUC1均呈阳性，4例MUC6呈阳性。这些黏蛋白的表达与肿瘤大小、组织学分级、淋巴结状态、雌激素受体状态、p53阳性或c-ErbB-2过表达之间未发现显著相关性。
结论
本研究记录了两种不同的黏蛋白（MUC5AC和MUC6）在少数乳腺癌以及正常和增生上皮中的表达，而正常乳腺组织未被描述为表达这两种黏蛋白。尽管黏蛋白在乳腺癌的恶性转化和进展中的作用尚未完全明了，但在某些情况下，可能存在几个编码不同黏蛋白的基因上调。
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