Wright J R, Frieden E
Bioinorg Chem. 1975 Jan;4(2):163-75. doi: 10.1016/s0006-3061(00)81025-x.
A characteristic red-violet chromophore which forms in the reaction of cupric ion with beta-sulfhydryl- alpha-amino acids has been attributed by several authors to a mixed-valence Cu3L2 structure. We have isolated and examined the analogous chromophore of the chelating drug penicillamine (beta, beta-dimethyl-cysteine), finding instead a polymeric anion with a mixed valence Cu2L2 repeating unit. Direct evidence for a mixed valence polymer cooroborates earlier conclusions of Wilson and Martin (9). Under oxygen-free conditions the anionic complex forms in essentially quantitative yield according to the following reaction: (see article). It has been established that this reaction is halide ion-dependent. The chromophore may be isolated as a sodium salt. This form was found to be stable, and it possessed no measurable activity as an oxidation catalyst. It was also demonstrated through biological experiments with rabbits that a significant amount of the complex- (between 6 percent and 39 percent) is recovered in the urinary tract following intravenous infusions attaining between 1.8 and 10 ppm copper.
几位作者认为，铜离子与β-巯基-α-氨基酸反应中形成的特征性红紫色发色团归因于混合价态的Cu3L2结构。我们分离并研究了螯合药物青霉胺（β，β-二甲基半胱氨酸）的类似发色团，结果发现是具有混合价态Cu2L2重复单元的聚合阴离子。混合价态聚合物的直接证据证实了威尔逊和马丁（9）早期的结论。在无氧条件下，阴离子络合物基本上以定量产率根据以下反应形成：（见文章）。已经确定该反应依赖于卤离子。发色团可以分离为钠盐。发现这种形式是稳定的，并且作为氧化催化剂没有可测量的活性。通过对兔子的生物学实验还表明，静脉输注达到1.8至10 ppm铜后，大量的络合物（6％至39％）在尿路中被回收。
