Gergely A, Sóvágó I
Bioinorg Chem. 1978 Jul;9(1):47-60. doi: 10.1016/s0006-3061(00)82005-0.
Complex formation and redox reactions between copper (II) ion and D-penicillamine were studied in detail as functions of the metal/-ligand ratio and the concentration of halide ions. It was established that a copper (I)- D-penicillamine polymeric complex of amphoteric character is formed when excess D-penicillamine is present. When the D-penicillamine/copper (II) ratio = 1.45 in the starting reaction mixture, a mixed valence complex with an intense red-violet color is formed. The formation of this compound, which contains 44% copper (II) ion, is greatly influenced by the experimental conditions, primarily by the concentration of halide ions. The main chemical and physical characteristics of the mixed valence complex were determined via magnetic and spectroscopic measurements. It was further established that a very intense blue complex is formed when the D-penicillamine/copper (II) ratio = 2 and halide ions are present. On the basis of the nature of the products formed under various conditions it was concluded that the copper (II)-D-penicillamine system may serve as a good model for studying the binding sites of copper-containing proteins.
详细研究了铜（II）离子与D-青霉胺之间的络合物形成及氧化还原反应，考察了其作为金属/配体比例和卤离子浓度的函数关系。已确定当存在过量的D-青霉胺时，会形成具有两性特征的铜（I）-D-青霉胺聚合物络合物。当起始反应混合物中D-青霉胺/铜（II）的比例 = 1.45时，会形成一种具有强烈红紫色的混合价络合物。该化合物含有44%的铜（II）离子，其形成受到实验条件的极大影响，主要是卤离子的浓度。通过磁性和光谱测量确定了混合价络合物的主要化学和物理特性。进一步确定当D-青霉胺/铜（II）的比例 = 2且存在卤离子时，会形成一种非常强烈的蓝色络合物。基于在各种条件下形成的产物的性质，得出结论：铜（II）-D-青霉胺体系可作为研究含铜蛋白质结合位点的良好模型。
