Yang Min, Wang Quankai, Wang Yawen, Li Ying
National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Wei Sheng Yan Jiu. 2007 Jul;36(4):404-6, 413.
To investigate the effect of glycidyl methacrylate (GMA) on cell cycle and apoptosis in human bronchial epithelial cells.
Flow cytometry was applied to determine cell cycle and apoptosis of 16HBE cells treated at the doses of 1-16 microg/ml GMA for different time. Mitotic index (MI) of cell was also analysed.
When 16HBE cells were treated by GMA for 72hr, cells in G0/G1 phase decreased, but cells in S and G2/M phase increased. The increases of apoptosis rates and the decreases of mitotic index could also be observed. As treatment went on, all groups cells were stopped at G0/G1 phase, but at the dose of 16 microg/ml the cell increases of S and G2/M phase were observed in comparison with the controls. After cells were treated for 3 times, the decreases apoptosis rates and the increases of mitotic index of cell treated by high dosage were observed.
The 16HBE cells treated by GMA could result in cell cycle transition from G0/G1 phase to S and G2/M phase. It was suggested that the cell proliferation was stimulated. Uncontrolled cellular proliferation may be responsible for malignant transformation induced by GMA.
研究甲基丙烯酸缩水甘油酯（GMA）对人支气管上皮细胞细胞周期及细胞凋亡的影响。
采用流式细胞术检测不同时间用1～16μg/ml GMA处理的16HBE细胞的细胞周期及细胞凋亡情况，并分析细胞的有丝分裂指数（MI）。
16HBE细胞经GMA处理72小时后，G0/G1期细胞减少，S期和G2/M期细胞增加，同时可观察到凋亡率升高和有丝分裂指数降低。随着处理时间延长，各处理组细胞均停滞于G0/G1期，但16μg/ml剂量组与对照组相比，S期和G2/M期细胞仍有增加。细胞经3次处理后，高剂量组细胞凋亡率降低，有丝分裂指数升高。
GMA处理16HBE细胞可导致细胞周期从G0/G1期向S期和G2/M期转变，提示GMA可刺激细胞增殖，细胞增殖失控可能是GMA诱导恶性转化的原因。
