Barnes F L, Sirard M A
IVF Labs, L.L.C., 11333 Moorpark, Studio City, CA 91602, USA.
Semin Reprod Med. 2000;18(2):123-31. doi: 10.1055/s-2000-12551.
The oocyte is dependent on granulosa cells to provide nutrients and regulatory signals. Granulosa cells must be at the appropriate stage of differentiation to initiate these signals and transmit them to the oocyte. Studies have shown that in vitro-matured oocytes from follicles in early stages of atresia are more competent to support embryonic development than those from actively growing follicles. The acquisition of developmental competence appears to occur prior to in vitro maturation and can be induced by gonadotropin-free coasting in vivo or postmortem ovary incubation in vitro. The acquisition of developmental competence is probably a common signaling or differentiation pathway that occurs in the oocyte and/or associated granulosa regardless of whether the oocyte is destined to ovulate or degenerate. Early follicle atresia is the visually discernible characteristic in vitro that is associated with increased developmental potential.
卵母细胞依赖颗粒细胞提供营养和调节信号。颗粒细胞必须处于适当的分化阶段才能启动这些信号并将其传递给卵母细胞。研究表明，来自闭锁早期卵泡的体外成熟卵母细胞比来自活跃生长卵泡的卵母细胞更有能力支持胚胎发育。发育能力的获得似乎发生在体外成熟之前，并且可以通过体内无促性腺激素的延缓或体外死后卵巢培养来诱导。无论卵母细胞注定要排卵还是退化，发育能力的获得可能是卵母细胞和/或相关颗粒细胞中发生的常见信号传导或分化途径。早期卵泡闭锁是体外可见的特征，与发育潜力增加有关。
