Maffei E M, Pompolo S G, Silva-Junior J C, Caixeiro A P, Rocha M P, Dergam J A
Departamento de Biologia Geral, Universidade Federal de Viçosa, Minas Gerais, Brasil.
Cytobios. 2001;104(406):119-25.
Adaptations of the nucleolar organizer regions (NOR) banding technique using precipitation of silver salts significantly improved the NOR characterization of some species of hymenopterans and one coleopteran. The bee Melipona marginata (2n = 18) showed one metacentric pair of chromosomes with a NOR in the pericentromeric position. The parasitic wasp Mellitobia australica (2n = 12) also showed one metacentric pair with a strongly Ag-positive NOR. The male lady-beetle Cycloneda sanguinea (2n = 18 + Xy(p)) displayed a NOR on a pair of acrocentric autosomes. In the male Euglossa sp. (a haplodiploid species) (n = 21) the NOR were multiple, and occurred in five chromosomes. In the bee Plebeia sp. 1 (2n = 34) the NOR seemed restricted to one of the homologues of a metacentric pair. The systematic advances brought out by using this technique in the context of current theories of karyotypic evolution of these taxa are described and discussed.
利用银盐沉淀法对核仁组织区（NOR）显带技术进行改进，显著提升了膜翅目某些物种和一种鞘翅目昆虫的NOR特征描述。蜜蜂Melipona marginata（2n = 18）显示一对中着丝粒染色体，NOR位于着丝粒周围位置。寄生蜂Mellitobia australica（2n = 12）也显示一对中着丝粒染色体，NOR呈强银阳性。雄性瓢虫Cycloneda sanguinea（2n = 18 + Xy(p)）在一对近端着丝粒常染色体上显示一个NOR。在雄性 Euglossa sp.（一种单倍二倍体物种）（n = 21）中，NOR是多个，存在于五条染色体上。在蜜蜂Plebeia sp. 1（2n = 34）中，NOR似乎局限于一对中着丝粒染色体的其中一条同源染色体上。本文描述并讨论了在这些分类单元的核型进化当前理论背景下使用该技术所带来的系统进展。
