Shangguan D, Han H, Zhao R, Zhao Y, Xiong S, Liu G
Center for Molecular Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing.
J Chromatogr A. 2001 Mar 2;910(2):367-72. doi: 10.1016/s0021-9673(00)01208-5.
A novel pre-column derivatization method for the quantitative determination of ginsenosides by HPLC with fluorescence detection was established. The double bond at the C24-C25 position of ginsenoside was converted into an aldehyde group by means of ozonolysis. Then the aldehyde group reacts with FMOC-hydrazine forming the ginsenoside FMOC-hydrazone. The derivatized products were separated by RP-HPLC with gradient elution. The detection limits of ginsenosides Rg1 and Rb1 were 2.0 ng (about 2.5 pmol) and 1.0 ng (about 0.9 pmol), respectively. This method can be used for all ginsenosides having the C24-C25 double bond.
建立了一种用于高效液相色谱荧光检测法定量测定人参皂苷的新型柱前衍生化方法。人参皂苷C24 - C25位的双键通过臭氧分解转化为醛基。然后醛基与芴甲氧羰基肼反应形成人参皂苷芴甲氧羰基腙。衍生化产物通过反相高效液相色谱梯度洗脱进行分离。人参皂苷Rg1和Rb1的检测限分别为2.0 ng（约2.5 pmol）和1.0 ng（约0.9 pmol）。该方法可用于所有具有C24 - C25双键的人参皂苷。
