Ignatov S G, Ferguson J A, Walt D R
State Research Center for Applied Microbiology, Obolensk, Moscow Region, Russia.
Biosens Bioelectron. 2001 Jan;16(1-2):109-13. doi: 10.1016/s0956-5663(00)00144-5.
A new type of fiber-optic biosensor based on bacterial cytoplasmic membranes (CPM) as the biological recognition element and an oxygen sensitive dye layer as the transducer is described for the detection of lactate. CPMs from bacteria with an induced lactate oxidase system are adsorbed onto a cellulose disk. The disk is fixed mechanically over an oxygen sensitive siloxane layer on the distal end of an optical fiber. This system detects lactate with no interference from glucose, fructose or glutamic acid.
描述了一种新型的光纤生物传感器，它以细菌细胞质膜（CPM）作为生物识别元件，以氧敏染料层作为换能器，用于检测乳酸。具有诱导型乳酸氧化酶系统的细菌的CPM被吸附到纤维素盘上。该盘被机械固定在光纤远端的氧敏硅氧烷层上方。该系统检测乳酸时不受葡萄糖、果糖或谷氨酸的干扰。
