Trettnak W, Wolfbeis O S
Analytical Division, Karl-Franzens-University, Graz, Austria.
Anal Biochem. 1990 Jan;184(1):124-7. doi: 10.1016/0003-2697(90)90023-3.
A biosensor for the continuous optical determination of cholesterol is presented. Cholesterol oxidase is immobilized covalently on a nylon membrane and the consumption of oxygen is measured by following, via fiberoptic bundles, the changes in fluorescence of an oxygen-sensitive dye whose fluorescence is dynamically quenched by molecular oxygen. The dye is dissolved in a very thin silicone membrane placed beneath the enzyme layer. During interaction of the enzyme with cholesterol, oxygen is consumed, which is indicated by the fluorescent dye. At pH 7.25, the analytical range of the sensor is 0.2 to 3 mM and the time to reach a full steady state in a flowing solution ranges from 7 to 12 min.
介绍了一种用于连续光学测定胆固醇的生物传感器。胆固醇氧化酶通过共价键固定在尼龙膜上，通过光纤束跟踪一种对氧敏感的染料的荧光变化来测量氧气的消耗，该染料的荧光被分子氧动态猝灭。该染料溶解在置于酶层下方的非常薄的硅树脂膜中。在酶与胆固醇相互作用期间，氧气被消耗，这由荧光染料指示。在pH 7.25时，该传感器的分析范围为0.2至3 mM，在流动溶液中达到完全稳定状态的时间为7至12分钟。
