Gloddek B, Harris J P
Division of Otolaryngology/Head and Neck Surgery, University of California, San Diego Medical Center.
Acta Otolaryngol. 1989 Jul-Aug;108(1-2):68-75. doi: 10.3109/00016488909107394.
In secondary inner ear responses against keyhole limpet hemocyanin (KLH), the characterization of interleukin-2 levels in perilymph and serum developing over 5 days in Hartley guinea pigs was investigated using an immunoassay. No interleukin-2 levels were detectable at Day 0 in serum and perilymph. The earliest perilymph IL-2 levels were observed after 6 h, peaking at 18 h and decreasing to zero by Day 5. In contrast, during this observation period, no IL-2 was detectable in serum or perilymph from control ears. Since previous work has identified T-helper cells in the endolymphatic sac, this site appears to be the probable source of interleukins. This study provides further support for the existence of an inner ear immune response which is regulated by lymphokines. Furthermore, interleukins may be a chemoattractant for cells entering the cochlea during the immune response.
在豚鼠针对钥孔戚血蓝蛋白（KLH）的继发性内耳反应中，采用免疫分析法研究了哈特利豚鼠外淋巴和血清中白细胞介素-2水平在5天内的变化特征。在第0天，血清和外淋巴中均未检测到白细胞介素-2水平。最早在外淋巴中检测到白细胞介素-2水平是在6小时后，18小时达到峰值，到第5天降至零。相比之下，在这个观察期内，对照耳的血清或外淋巴中未检测到白细胞介素-2。由于先前的研究已经在内淋巴囊中鉴定出辅助性T细胞，这个部位似乎是白细胞介素的可能来源。本研究进一步支持了存在由淋巴因子调节的内耳免疫反应。此外，白细胞介素可能是免疫反应期间进入耳蜗的细胞的趋化因子。
