藜芦生物碱对肾性高血压大鼠中枢儿茶酚胺能神经元的影响。
Effects of Veratrum nigrum alkaloids on central catecholaminergic neurons of renal hypertensive rats.
作者信息
Li H, Gao G Y, Li S Y, Zhao W J, Guo Y T, Liang Z J
机构信息
Department of Pharmacology, Dalian Medical University, Dalian 116027, China.
出版信息
Acta Pharmacol Sin. 2000 Jan;21(1):23-8.
AIM
To study the central hypotensive mechanism of Veratrum nigrum L var ussurience Nakai alkaloids (VnA) in renal hypertensive rats(RHR).
METHODS
The quantitative method of immunocytochemistry (ICC) was used to observe and detect the effect of VnA (30 micrograms.kg-1, i.v.) on activity of central catecholaminergic (CA) neurons of C1, C2, A1, and A5 areas in RHR.
RESULTS
VnA increased the immunoreactivity (IR) of tyrosine 3-monooxygenase (TM)-immunopositive (IP) neurons of C1, C2, and A5 areas in RHR experimental group compared with RHR control group [positive units: (1.9 +/- 0.4), (1.18 +/- 0.23), (1.2 +/- 0.4) vs (0.15 +/- 0.22), (0.31 +/- 0.16), (0.69 +/- 0.20), respectively]; IR of TM-IP neurons of C1 and C2 areas in RHR control group was decreased compared with sham-operated group [positive units: (0.15 +/- 0.22), (0.31 +/- 0.16) vs (1.45 +/- 0.29), (1.36 +/- 0.25), respectively].
CONCLUSION
VnA increased the activity of central CA neurons in RHR to exert its hypotensive effect.
目的
研究藜芦碱（VnA）对肾性高血压大鼠（RHR）的中枢降压机制。
方法
采用免疫细胞化学定量法（ICC）观察并检测VnA（30微克·千克-1，静脉注射）对RHR中C1、C2、A1和A5区中枢儿茶酚胺能（CA）神经元活性的影响。
结果
与RHR对照组相比，RHR实验组中VnA增加了C1、C2和A5区酪氨酸3-单加氧酶（TM）免疫阳性（IP）神经元的免疫反应性（IR）[阳性单位：分别为（1.9±0.4）、（1.18±0.23）、（1.2±0.4）对比（0.15±0.22）、（0.31±0.16）、（0.69±0.20）]；与假手术组相比，RHR对照组中C1和C2区TM-IP神经元的IR降低[阳性单位：分别为（0.15±0.22）、（0.31±0.16）对比（1.45±0.29）、（1.36±0.25）]。
结论
VnA增加RHR中枢CA神经元的活性以发挥其降压作用。
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