并非由RNA聚合酶II合成的信使RNA可以在3'端被切割并进行聚腺苷酸化。
Messenger RNAs that are not synthesized by RNA polymerase II can be 3' end cleaved and polyadenylated.
作者信息
Fodor E, Mikulasova A, Mingay L J, Poon L L, Brownlee G G
机构信息
Sir William Dunn School of Pathology, University of Oxford, UK.
出版信息
EMBO Rep. 2000 Dec;1(6):513-8. doi: 10.1093/embo-reports/kvd111.
The poly(A) tail of influenza virus mRNAs is synthesized by the viral RNA polymerase by reiterative copying of a U5-7 sequence near the 5' end of the viral RNA (vRNA) template. We have engineered a vRNA molecule by replacing its viral U6 poly(A) site with a negative-sense eukaryotic polyadenylation signal. The vRNA was transcribed by the viral RNA polymerase and the transcription product was processed by the cellular 3' end processing machinery in vivo. According to the current model, 3' end processing of eukaryotic pre-mRNAs is coupled to cellular RNA polymerase II (pol II) transcription; thus only RNAs synthesized by pol III are believed to be polyadenylated efficiently. Our results show that the cellular polyadenylation machinery is nevertheless able to recognize and process RNA transcripts that are not synthesized by pol II, indicating that synthesis by pol II is not an absolute requirement for 3' end processing in vivo.
流感病毒mRNA的聚腺苷酸尾是由病毒RNA聚合酶通过对病毒RNA（vRNA）模板5'端附近的U5-7序列进行反复复制而合成的。我们通过用负义真核聚腺苷酸化信号替换其病毒U6聚腺苷酸位点来构建了一个vRNA分子。该vRNA由病毒RNA聚合酶转录，转录产物在体内由细胞3'端加工机制进行加工。根据目前的模型，真核前体mRNA的3'端加工与细胞RNA聚合酶II（pol II）转录偶联；因此，只有由pol III合成的RNA被认为能有效地进行聚腺苷酸化。我们的结果表明，细胞聚腺苷酸化机制仍然能够识别和加工并非由pol II合成的RNA转录物，这表明pol II的合成并非体内3'端加工的绝对必要条件。
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