Kambe N, Kambe M, Kochan J P, Schwartz L B
Department of Internal Medicine, Division of Rheumatology, Allergy and Immunology, Virginia Commonwealth University, Richmond, Virginia 23298-0263, USA.
Blood. 2001 Apr 1;97(7):2045-52. doi: 10.1182/blood.v97.7.2045.
Human mast cells in adult tissues have been thought to have limited, if any, proliferative potential. The current study examined mast cells obtained from adult skin and cultured in serum-free medium with recombinant human stem cell factor. During the first 4 weeks of culture, the percentages of mast cells increased from 10 to almost 100. After 8 weeks, a 150-fold increase in the number of mast cells was observed. When freshly dispersed mast cells were individually sorted onto human fibroblast monolayers and cultured for 3 weeks, one or more mast cells were detected in about two thirds of the wells, and in about two thirds of these wells the surviving mast cells showed evidence of proliferation, indicating most mast cells in skin can proliferate. Such mast cells all expressed high surface levels of Kit and Fc epsilon RI, each of which were functional, indicating IgE was not required for Fc epsilon RI expression on mast cells. Such mast cells also retained the MC(TC) protease phenotype of mast cells that normally reside in the dermis. After 4 to 8 weeks of culture these mast cells degranulated in response to substance P and compound 48/80, characteristics of skin-derived mast cells that persist outside of the cutaneous microenvironment. (Blood. 2001;97:2045-2052)
成年组织中的人类肥大细胞一直被认为，即便有增殖潜力，也极为有限。当前研究检测了取自成人皮肤并在含重组人干细胞因子的无血清培养基中培养的肥大细胞。在培养的前4周，肥大细胞的百分比从10%增至近100%。8周后，观察到肥大细胞数量增加了150倍。当将刚分散的肥大细胞逐个分选到人类成纤维细胞单层上并培养3周时，约三分之二的孔中检测到一个或多个肥大细胞，并且在这些孔中的约三分之二，存活的肥大细胞显示出增殖迹象，表明皮肤中的大多数肥大细胞可以增殖。此类肥大细胞均高表达功能性的Kit和FcεRI表面水平，这表明肥大细胞上FcεRI的表达不需要IgE。此类肥大细胞还保留了正常存在于真皮中的肥大细胞的MC(TC)蛋白酶表型。培养4至8周后，这些肥大细胞对P物质和化合物48/80发生脱颗粒反应，这是皮肤来源的肥大细胞在皮肤微环境之外仍保持的特征。（《血液》。2001年；97:2045 - 2052）
