Holbrook M R, Wang H, Barrett A D
Center for Tropical Diseases and Department of Pathology, University of Texas Medical Branch, Galveston, Texas 77555-0609, USA.
J Virol. 2001 Apr;75(8):3999-4001. doi: 10.1128/JVI.75.8.3999-4001.2001.
Langat (LGT) virus M protein has been generated in a recombinant system. Antiserum raised against the LGT virus M protein neutralizes tick-borne encephalitis serocomplex flaviviruses but not mosquito-borne flaviviruses, indicating that the M protein is exposed on the surface of virions. The antiserum recognizes intracellular LGT virus prM/M and binds to prM and M in Western blots of whole-cell lysates and purified virus, respectively. These data suggest that the prM and M proteins are structurally similar under native conditions and support the hypothesis that the "pr" portion of prM facilitates proper folding of the M protein for expression on the virion surface.
兰加特（LGT）病毒M蛋白已在重组系统中产生。针对LGT病毒M蛋白产生的抗血清可中和蜱传脑炎血清复合群黄病毒，但不能中和蚊传黄病毒，这表明M蛋白暴露在病毒粒子表面。该抗血清可识别细胞内的LGT病毒prM/M，并分别在全细胞裂解物和纯化病毒的蛋白质印迹中与prM和M结合。这些数据表明，在天然条件下prM和M蛋白在结构上相似，并支持以下假设：prM的“pr”部分有助于M蛋白正确折叠以便在病毒粒子表面表达。
