Global Research and Development, Baxter BioScience, Vienna, Austria.
J Infect Dis. 2011 Jun 1;203(11):1556-64. doi: 10.1093/infdis/jir122.
After vaccination of humans with tick-borne encephalitis virus (TBEV) vaccine, the extent of cross-neutralization between viruses of the European, Far Eastern, and Siberian subtypes of TBEV and Omsk hemorrhagic fever virus (OHFV) was analyzed. Hybrid viruses that encode the TBEV surface proteins for representative viruses within all subtypes, and OHFV, were constructed using the West Nile virus (WNV) backbone as vector. These viruses allow for unbiased head-to-head comparison in neutralization assays because they exhibit the antigenic characteristics of the TBEV strains from which the surface proteins were derived and showed equivalent biologic properties in cell culture. Human serum samples derived from a TBEV vaccine trial were analyzed and revealed comparable neutralizing antibody titers against European, Far Eastern, and Siberian subtype viruses, indicating equally potent cross-protection against these TBEV strains and a somewhat reduced but still protective neutralization capacity against more distantly related viruses, such as OHFV.
在人类接种蜱传脑炎病毒（TBEV）疫苗后，分析了 TBEV 的欧洲、远东和西伯利亚亚型病毒与鄂木斯克出血热病毒（OHFV）之间交叉中和的程度。使用西尼罗河病毒（WNV）作为载体，构建了编码 TBEV 表面蛋白的杂交病毒，这些病毒代表了所有亚型和 OHFV 的代表性病毒。这些病毒允许在中和试验中进行无偏见的直接比较，因为它们表现出源自 TBEV 株的表面蛋白的抗原特征，并且在细胞培养中表现出相当的生物学特性。从 TBEV 疫苗试验中获得的人血清样本进行了分析，并显示出对欧洲、远东和西伯利亚亚型病毒的可比中和抗体滴度，表明对这些 TBEV 株具有同等有效的交叉保护作用，并且对距离较远的病毒（如 OHFV）的中和能力略有降低但仍具有保护作用。
