Du Toit M, Dicks L M, Holzapfel W H
Department of Microbiology, University of Stellenbosch, South Africa.
Lett Appl Microbiol. 2001 Mar;32(3):199-204. doi: 10.1046/j.1472-765x.2001.00889.x.
Identification and characterization of obligately homofermentative and facultatively heterofermentative strains of Lactobacillus spp. isolated from the faeces of pigs that had been raised under different conditions.
The phenotypic relatedness of the isolated strains and reference strains were determined by numerical analysis of total soluble cell protein patterns and simple physiological and biochemical tests. Of the 23 strains isolated from faeces, nine were obligately homofermentative and 14 facultatively heterofermentative. The strains clustered at r > or = 0.61 with Lactobacillus amylovorus (seven strains), Lactobacillus crispatus (one strain), Lactobacillus plantarum (14 strains) and Lactobacillus intestinalis (one strain).
Results obtained from the physiological and biochemical tests confirmed the identity of the isolates as determined by numerical analysis of total soluble cell protein profiles.
This is the first report on the association of Lact. crispatus and Lact. intestinalis with the gastro-intestinal tract of pigs.
鉴定和表征从不同饲养条件下猪的粪便中分离出的专性同型发酵和兼性异型发酵乳酸杆菌菌株。
通过对总可溶性细胞蛋白模式进行数值分析以及简单的生理生化试验，确定分离菌株与参考菌株的表型相关性。从粪便中分离出的23株菌株中，9株为专性同型发酵菌，14株为兼性异型发酵菌。这些菌株在相关系数r≥0.61时，与解淀粉乳酸杆菌（7株）、卷曲乳酸杆菌（1株）、植物乳杆菌（14株）和肠道乳酸杆菌（1株）聚类在一起。
生理生化试验结果证实了通过总可溶性细胞蛋白谱数值分析所确定的分离株的身份。
这是关于卷曲乳酸杆菌和肠道乳酸杆菌与猪胃肠道关联的首次报道。
