Du Toit M, Dicks L M T, Holzapfel W H
Department of Microbiology, Stellenbosch University, Private Bag X1, Matieland ZA-7602 South Africa.
Lett Appl Microbiol. 2003;37(1):12-6. doi: 10.1046/j.1472-765x.2003.01334.x.
The study deals with a number of heterofermentative Lactobacillus strains isolated from pig faeces and their identification.
SDS-PAGE of total soluble cell proteins and RAPD-PCR profiles were used to identify the strains isolated from pig faeces. Protein profiles obtained with SDS-PAGE revealed that 15 strains clustered at r >or= 0.78 with Lactobacillus buchneri and nine strains at r >or= 0.77 with two reference strains of Lactobacillus reuteri. The identity of the strains was confirmed with RAPD-PCR.
Numerical analysis of protein profiles and RAPD-PCR proved valuable in the differentiation of Lactobacillus spp. isolated from pig faeces.
This is the first report on the association of Lact. buchneri with pig faeces.
本研究涉及从猪粪便中分离出的多种异型发酵乳酸菌菌株及其鉴定。
采用全可溶性细胞蛋白的SDS-PAGE和RAPD-PCR图谱对从猪粪便中分离出的菌株进行鉴定。SDS-PAGE获得的蛋白质图谱显示，15株菌株与布氏乳杆菌在r≥0.78时聚类，9株菌株与两株罗伊氏乳杆菌参考菌株在r≥0.77时聚类。通过RAPD-PCR证实了这些菌株的身份。
蛋白质图谱和RAPD-PCR的数值分析在从猪粪便中分离的乳杆菌属菌种鉴别中证明是有价值的。
这是关于布氏乳杆菌与猪粪便关联的首次报道。
