Molin G, Jeppsson B, Johansson M L, Ahrné S, Nobaek S, Ståhl M, Bengmark S
Department of Food Technology, Lund University, Sweden.
J Appl Bacteriol. 1993 Mar;74(3):314-23. doi: 10.1111/j.1365-2672.1993.tb03031.x.
Two-hundred and fifty Lactobacillus strains isolated from healthy and diseased mucosa of human intestines of 75 individuals and 49 reference strains were phenotypically classified using 49 unit characters. Data were processed by the Jaccard (SJ) and Simple Matching (SSM) coefficients, and unweighted pair group algorithm with arithmetic averages. Seventeen major clusters were defined at the 76% SJ-similarity level which approximately correspond to the SSM-level of 91%. Seven clusters could be identified: Lactobacillus plantarum (isolates recovered from 5% of the patients), Lact. casei subsp. rhamnosus (17% of the patients), Lact. casei subsp. pseudoplantarum (5% of the patients), Leuconostoc mesenteroides subsp. mesenteroides (3% of the patients), Lact. buchneri (4% of patients), Lact. reuteri (4% of the patients) and Lact. salivarius subsp. salivarius (9% of the patients). Unassigned clusters 1 and 3 both contained homofermentative Lactobacillus strains. Cluster 1 included the type strains of Lact. crispatus, Lact. acidophilus, Lact. jensenii and Lact. gasseri, and cluster 3, the type strains of Lact. delbrueckii subsp. lactis, Lact. agilis and Lact. casei subsp. tolerans. Clusters 1 and 3 were found in 15% and 25% of the patients, respectively. Unassigned clusters 2, 6, 7, 8 and 10 contained homofermentative Lactobacillus strains but no reference strains. Clusters 11, 12, 15 and 17 were made up of heterofermentative Lactobacillus strains but no reference strains. Phenotypical characteristics of the clusters are given. No obvious trends in species (cluster) composition between different intestinal locations could be noted. Most clusters contained isolates from both diseased and healthy mucosa. Exceptions were cluster 15 and cluster 17 which only included isolates from healthy mucosa, and cluster 11 which only included isolates from diseased mucosa. Cluster 15 was isolated in 12% of the patients, and cluster 11 in 8%.
从75名个体的健康和患病肠道黏膜中分离出250株乳酸杆菌菌株，并使用49个单位性状对49株参考菌株进行了表型分类。数据通过雅卡尔（SJ）系数和简单匹配（SSM）系数以及算术平均的非加权配对组算法进行处理。在76%的SJ相似性水平上定义了17个主要聚类，这大约对应于91%的SSM水平。可以识别出7个聚类：植物乳杆菌（从5%的患者中分离得到）、干酪乳杆菌鼠李糖亚种（17%的患者）、干酪乳杆菌假植物亚种（5%的患者）、肠膜明串珠菌肠膜亚种（3%的患者）、布氏乳杆菌（4%的患者）、罗伊氏乳杆菌（4%的患者）和唾液乳杆菌唾液亚种（9%的患者）。未分类的聚类1和3都包含同型发酵乳酸杆菌菌株。聚类1包括卷曲乳杆菌、嗜酸乳杆菌、詹氏乳杆菌和加氏乳杆菌的模式菌株，聚类3包括德氏乳杆菌乳酸亚种、敏捷乳杆菌和干酪乳杆菌耐酸亚种的模式菌株。聚类1和3分别在15%和25%的患者中被发现。未分类的聚类2、6、7、8和10包含同型发酵乳酸杆菌菌株但没有参考菌株。聚类11、12、15和17由异型发酵乳酸杆菌菌株组成但没有参考菌株。给出了各聚类的表型特征。在不同肠道部位之间未发现明显的物种（聚类）组成趋势。大多数聚类包含来自患病和健康黏膜的分离株。例外情况是聚类15和聚类17仅包括来自健康黏膜的分离株，聚类11仅包括来自患病黏膜的分离株。12%的患者中分离出聚类15，8%的患者中分离出聚类11。
