Wyse C A, Murphy D M, Preston T, Morrison D J, Love S
Department of Veterinary Clinical Studies, University of Glasgow Veterinary School, Bearsden, UK.
Equine Vet J. 2001 Mar;33(2):197-203. doi: 10.1111/j.2042-3306.2001.tb00601.x.
The aim of this study was to assess the feasibility of applying the 13C-octanoic acid breath test for assessment of gastric emptying in ponies by investigating the pattern of 13C enrichment in breath following the administration of a test meal +/- 13C-octanoic acid. After a 14 h fast, the ponies received either no meal (Test I) or a standardised test meal labelled with 0 mg (Test II), 125 mg (Test III), 250 mg (Test IV) or 500 mg (Test V) 13C-octanoic acid. For each test (I-V), exhaled breath samples were collected in duplicate at 1 h and immediately before ingestion of the test meal and at frequent intervals thereafter for 12 h. Breath samples were analysed by continuous flow isotope ratio mass spectrometry. Three indices of breath 13C-enrichment were computed; half dose recovery time (t1/2), gastric emptying coefficient (GEC) and time to peak breath 13C-enrichment t(max). For Tests I and II, the ratio of 13CO2:12CO2 remained stable for the duration of the sampling period. For Tests III, IV and V, an increase in the ratio of 13CO2:12CO2 was detected. The test was reproducible within individuals, and intersubject variation was low. Further validation studies of this noninvasive technique are justified.
本研究的目的是通过研究给予试验餐±13C-辛酸后呼出气体中13C富集模式，评估应用13C-辛酸呼气试验评估小马胃排空的可行性。禁食14小时后，小马分别接受无餐（试验I）或标记有0毫克（试验II）、125毫克（试验III）、250毫克（试验IV）或500毫克（试验V）13C-辛酸的标准化试验餐。对于每个试验（I-V），在摄入试验餐前1小时和立即摄入试验餐时，以及此后12小时内每隔一段时间重复采集呼出气体样本。通过连续流动同位素比率质谱法分析呼出气体样本。计算了呼出气体13C富集的三个指标；半剂量恢复时间（t1/2）、胃排空系数（GEC）和呼出气体13C富集达到峰值的时间t(max)。对于试验I和II，在采样期间13CO2:12CO2的比率保持稳定。对于试验III、IV和V，检测到13CO2:12CO2的比率增加。该试验在个体内具有可重复性，且个体间差异较小。对这种非侵入性技术进行进一步的验证研究是合理的。
