Wyse C A, Murphy D M, Preston T, Sutton D G, Morrison D J, Christley R M, Love S
Department of Veterinary Clinical Studies, University of Glasgow Veterinary School, Bearsden, Glasgow, G61 1QH. 9707285
Res Vet Sci. 2001 Aug;71(1):81-3. doi: 10.1053/rvsc.2001.0488.
The aim of this study was to apply the(13)C-octanoic acid breath test for detection of alterations in the rate of solid-phase gastric emptying, induced by changes in test meal composition, in ponies. After a 14 hour fast the ponies (n = 4) ingested a test meal with 0, 35 or 70 ml soya oil, and labelled with 250 mg(13)C-octanoic acid. Each pony was given each of the three test meals on three separate occasions, in a randomised order. Exhaled breath samples were collected for 12 hours after ingestion of the test meal. Breath samples were analysed by continuous flow isotope ratio mass spectrometry. Three indices of breath(13)C-enrichment were computed, half-dose recovery time (t 1/2), gastric emptying coefficient (GEC) and time to peak breath(13)C-excretion t(max). The(13)C-octanoic acid breath test was a reliable means of assessing the significantly decreased rate of gastric emptying in the pony, associated with addition of soya oil to the test meal.
本研究的目的是应用¹³C-辛酸呼气试验，检测在小马中，由试验餐成分变化引起的固相胃排空速率的改变。禁食14小时后，小马（n = 4）摄入含有0、35或70毫升大豆油且标记有250毫克¹³C-辛酸的试验餐。每匹小马在三个不同的时间以随机顺序分别接受这三种试验餐。试验餐摄入后收集12小时的呼气样本。呼气样本通过连续流动同位素比率质谱法进行分析。计算了呼气¹³C富集的三个指标，半剂量恢复时间（t 1/2）、胃排空系数（GEC）和呼气¹³C排泄峰值时间t（max）。¹³C-辛酸呼气试验是评估小马胃排空速率显著降低的可靠方法，这种降低与试验餐中添加大豆油有关。
