Fang S, Yu J, Feng J, Han C, Li D, Liu Y
National Laboratories for Agrobiotechnology, China Agricultural University, Beijing.
Arch Virol. 2001;146(1):167-70. doi: 10.1007/s007050170200.
Three virus isolates from maize with rough dwarf in different provinces in China were analyzed at the molecular level. When compared to an isolate from diseased rice plants in Hubei Province, all four isolates had identical genomic RNA electrophoretic profiles, which were composed of ten double-stranded (ds) RNAs. Full-length cDNAs of segment 10 (S10) from each of the four isolates were cloned by RT-PCR and the complete sequences were determined. Analysis of the sequences revealed that each consisted of 1801 nucleotides and contained a single open reading frame (ORF) which potentially encoded a protein with 558 amino acids. Further, the sequences showed more than 97.0% and 98.0% identity at nucleotide and amino acid levels, respectively. In addition, their identities to rice black-streaked dwarf virus S10 were significantly higher than those to maize rough dwarf virus S10. Based on these results, it is suggested that the virus which causes this maize disease in China is rice black-streaked dwarf virus.
对来自中国不同省份的3株玉米粗缩病病毒分离物进行了分子水平分析。与湖北省水稻病株上的一株分离物相比，这4株分离物的基因组RNA电泳图谱完全相同，均由10条双链（ds）RNA组成。通过RT-PCR克隆了这4株分离物第10节段（S10）的全长cDNA，并测定了其完整序列。序列分析表明，每个序列由1801个核苷酸组成，包含一个单一的开放阅读框（ORF），该阅读框可能编码一个含有558个氨基酸的蛋白质。此外，这些序列在核苷酸和氨基酸水平上的同一性分别超过97.0%和98.0%。此外，它们与水稻黑条矮缩病毒S10的同一性显著高于与玉米粗缩病毒S10的同一性。基于这些结果，表明在中国引起这种玉米病害的病毒是水稻黑条矮缩病毒。
