Bai Feng-Wei, Yan Jian, Qu Zhi-cai, Zhang Hong-Wei, Xu Jia, Ye Ming-Ming, Shen Da-leng
Institute of Genetics, School of Life Sciences, Fudan University, Shanghai, People's Republic of China.
Virus Genes. 2002 Oct;25(2):201-6. doi: 10.1023/a:1020170020581.
A viral disease with dwarfing symptoms is associated with severe damage of different cereal crops including rice, maize, wheat and sorghum grown in China. It is believed that the pathogenic agent of the disease on rice and sorghum is rice black streaked dwarf virus (RBSDV), however, the cause of maize dwarf disease in China is still inconclusive. In this report, dsRNA was isolated from virus particles obtained from the diseased plants of rice, maize, wheat and sorghum from two Chinese provinces. Full-length cDNAs of genome segments 9 (S9) and 10 (S 10) were obtained through a RT-PCR approach. Sequence analysis showed that the S9 sequences of Chinese isolates and Japanese RBSDV isolate were very similar to each other (89.1-89.6% identity at the nucleotide level, 92.3-92.9% and 95.8-98.6% identity at the amino acid level for ORF1 and ORF2, respectively). In addition, the S10 sequences of Chinese isolates and Japanese RBSDV were very similar to each other (93.0-95.4% identical nucleotides and 96.2-97.0% identical amino acids, respectively). However, there were lower similarities for S9 and S10 sequences between Chinese isolates and an Italian Maize Rough Dwarf Virus (MRDV) isolate. Phylogenetic analysis indicates that Chinese viral isolates found to infect rice, maize, wheat and sorghum and leading to similar cereal dwarfing manifestations could be grouped to the same virus species, RBSDV.
一种伴有矮化症状的病毒性疾病与包括中国种植的水稻、玉米、小麦和高粱在内的不同谷类作物的严重损害有关。据信，水稻和高粱上该疾病的病原体是水稻黑条矮缩病毒（RBSDV），然而，中国玉米矮缩病的病因仍无定论。在本报告中，从中国两个省份的水稻、玉米、小麦和高粱病株所获病毒颗粒中分离出双链RNA。通过逆转录聚合酶链反应（RT-PCR）方法获得了基因组片段9（S9）和10（S10）的全长互补DNA。序列分析表明，中国分离株和日本RBSDV分离株的S9序列彼此非常相似（核苷酸水平上的同一性为89.1 - 89.6%，开放阅读框1（ORF1）和开放阅读框2在氨基酸水平上的同一性分别为92.3 - 92.9%和95.8 - 98.6%）。此外，中国分离株和日本RBSDV的S10序列彼此非常相似（核苷酸同一性分别为93.0 - 95.4%，氨基酸同一性分别为96.2 - 97.0%）。然而，中国分离株与意大利玉米粗缩病毒（MRDV）分离株的S9和S10序列之间的相似性较低。系统发育分析表明，在中国发现的感染水稻、玉米、小麦和高粱并导致类似谷类作物矮化表现的病毒分离株可归为同一病毒种类，即RBSDV。
