Vilcek S, Paton D J, Durkovic B, Strojny L, Ibata G, Moussa A, Loitsch A, Rossmanith W, Vega S, Scicluna M T, Paifi V
University of Veterinary Medicine, Kosice, Slovakia.
Arch Virol. 2001;146(1):99-115. doi: 10.1007/s007050170194.
Seventy-eight bovine viral diarrhoea viruses (BVDV) recently collected in Austria, France, Hungary, Italy, Slovakia, Spain and UK were genetically typed in the 5'-untranslated (5'UTR) and autoprotease (Npro) regions of the pestivirus genome. Seventy-six of the isolates were BVDV-1 and two French isolates were of the BVDV-2 genotype. Phylogenetic analysis of the 5'UTR (245 nt), including additional BVDV-1 sequences from USA, Canada, Germany, New Zealand, Mozambique and Sweden, taken from GenBank and from our previous works, indicated that these viruses were clustered not only into the two generally accepted groups (BVDV-1a-"NADL like" and BVDV-1b-"Osloss like"), but altogether into 11 phylogenetic groups. Similar clustering was observed with Npro region sequences (385 nt) and the highest bootstrap values (over 95%) were obtained by phylogeny combining 5'UTR and Npro sequences. Some associations between the genetic grouping and the origin of the isolates were apparent, probably reflecting historical trade contacts. Considering the variability of isolates it is recommended that diagnostic PCR primers should be re-examined to ensure coverage of all BVDV-1 groups. The genogroups were less clearly differentiated by monoclonal antibody typing, suggesting significant antigenic similarities within the BVDV-1 genotype.
最近在奥地利、法国、匈牙利、意大利、斯洛伐克、西班牙和英国收集的78株牛病毒性腹泻病毒（BVDV）在瘟病毒基因组的5'-非翻译区（5'UTR）和自蛋白酶（Npro）区域进行了基因分型。其中76株分离株为BVDV-1型，两株法国分离株为BVDV-2基因型。对5'UTR（245个核苷酸）进行系统发育分析，包括从GenBank以及我们之前的研究中获取的来自美国、加拿大、德国、新西兰、莫桑比克和瑞典的额外BVDV-1序列，结果表明这些病毒不仅聚类为两个普遍认可的组（BVDV-1a - “NADL样”和BVDV-1b - “Osloss样”），而且总共聚类为11个系统发育组。在Npro区域序列（385个核苷酸）中也观察到类似的聚类情况，并且通过结合5'UTR和Npro序列进行系统发育分析获得了最高的自展值（超过95%）。分离株的基因分组与其来源之间存在一些明显的关联，这可能反映了历史贸易联系。考虑到分离株的变异性，建议重新审视诊断性PCR引物，以确保覆盖所有BVDV-1组。通过单克隆抗体分型对基因群的区分不太明显，这表明在BVDV-1基因型内存在显著的抗原相似性。
