Abounaaja Fatima, Babaoglu Ali Riza
Department of Virology, Institute of Health Sciences, Van Yuzuncu Yil University, Van, Turkey.
Department of Virology, Faculty of Veterinary Medicine, Van Yuzuncu Yil University, Van, Turkey.
Vet Med Sci. 2025 Jan;11(1):e70127. doi: 10.1002/vms3.70127.
Bovine viral diarrhoea virus (BVDV) infection, caused by Pestiviruses A and B, with various clinical findings and causes significant economic losses. This disease is common in Turkey as well as in other countries, especially in European countries.
This study was designed to determine the genotypes of BVDVs and their variability among cattle in eastern Turkey.
A total of 110 samples from 85 cattle suspected of BVDV infection were tested using RT-PCR with primers targeting the 5'UTR, autoprotease (N) and E2 gene regions of pestiviruses. Sequence and phylogenetic analyses were performed on the 5'UTR and N gene regions of these samples.
Analysis of 15 sequences obtained from 13 cattle revealed that Pestivirus A (BVDV-1) was responsible for the infection. In addition, the study identified subgenotypes BVDV-1a (n = 5), 1b (n = 5), 1d (n = 1), 1f (n = 1), 1l (n = 1) and 1r (n = 2). No evidence of infection with Pestivirus B (BVDV-2), Pestivirus D (Border disease virus) or Pestivirus H (HoBi-like virus/BVDV-3) was found.
The significance of pestiviruses in causing genital and respiratory problems is once again emphasised, underscoring the necessity of including them in herd screening. Identifying BVDV genetic diversity both in Turkey and worldwide is crucial for developing effective protection, control and eradication strategies, particularly for vaccination programs. As a conclusion, the identification of BVDV-1a, 1b, 1d, 1f, 1l and 1r in the eastern provinces of Turkey points to an increase in BVDV-1 genetic diversity.
牛病毒性腹泻病毒（BVDV）感染由A和B型瘟病毒引起，具有多种临床表现，并造成重大经济损失。这种疾病在土耳其以及其他国家很常见，尤其是在欧洲国家。
本研究旨在确定土耳其东部牛群中BVDV的基因型及其变异性。
使用针对瘟病毒5'非翻译区（UTR）、自蛋白酶（N）和E2基因区域的引物，通过逆转录聚合酶链反应（RT-PCR）对来自85头疑似感染BVDV的牛的110份样本进行检测。对这些样本的5'UTR和N基因区域进行序列和系统发育分析。
对从13头牛获得的15个序列的分析表明，感染是由A型瘟病毒（BVDV-1）引起。此外，该研究鉴定出BVDV-1a（n = 5）、1b（n = 5）、1d（n = 1）、1f（n = 1）、1l（n = 1）和1r（n = 2）亚基因型。未发现感染B型瘟病毒（BVDV-2）、D型瘟病毒（边界病病毒）或H型瘟病毒（类HoBi病毒/BVDV-3）的证据。
再次强调了瘟病毒在引起生殖和呼吸问题方面的重要性，突出了将它们纳入畜群筛查的必要性。识别土耳其和全球范围内的BVDV遗传多样性对于制定有效的保护、控制和根除策略至关重要，特别是对于疫苗接种计划。总之，在土耳其东部省份鉴定出BVDV-1a、1b、1d、1f、1l和1r表明BVDV-1的遗传多样性增加。
