Jayasinghe S, Hristova K, White S H
Department of Physiology and Biophysics, University of California, Irvine, California 92697-4560, USA.
Protein Sci. 2001 Feb;10(2):455-8. doi: 10.1110/ps.43501.
The reliability of the transmembrane (TM) sequence assignments for membrane proteins (MPs) in standard sequence databases is uncertain because the vast majority are based on hydropathy plots. A database of MPs with dependable assignments is necessary for developing new computational tools for the prediction of MP structure. We have therefore created MPtopo, a database of MPs whose topologies have been verified experimentally by means of crystallography, gene fusion, and other methods. Tests using MPtopo strongly validated four existing MP topology-prediction algorithms. MPtopo is freely available over the internet and can be queried by means of an SQL-based search engine.
标准序列数据库中膜蛋白（MP）跨膜（TM）序列分配的可靠性尚不确定，因为绝大多数是基于亲水性图谱。对于开发预测MP结构的新计算工具而言，一个具有可靠分配的MP数据库是必要的。因此，我们创建了MPtopo，这是一个MP数据库，其拓扑结构已通过晶体学、基因融合和其他方法进行了实验验证。使用MPtopo进行的测试有力地验证了四种现有的MP拓扑预测算法。MPtopo可通过互联网免费获取，并可通过基于SQL的搜索引擎进行查询。
