El-Helow E R
Department of Botany, Faculty of Science, University of Alexandria, Alexandria 21526, Egypt.
FEMS Microbiol Lett. 2001 Mar 15;196(2):119-22. doi: 10.1111/j.1574-6968.2001.tb10551.x.
A Tween-80-degrading novel marine Bacillus strain, N10, has recently been isolated in Alexandria University, Egypt. The taxonomic position of this endospore forming bacterium was investigated on the basis of fatty acid analysis and 16S rRNA gene sequencing. Comparative computer database analyses revealed that the bacterium is a Bacillus subtilis strain. The gene encoding the small acid-soluble protein gamma-type (SASP-B), sspE, was successfully utilized in this study as a tool for discrimination between the two B. subtilis subspecies W23 and 168. Based on the alignment of 16S rRNA sequences and analysis of SASP-B relatedness, it has been demonstrated that the novel marine B. subtilis strain N10 is more closely related to the B. subtilis reference strain W23 than to 168. The strain, N10, has been deposited in the Bacillus Genetic Stock Center (BGSC) and assigned the accession number 3A17.
最近，埃及亚历山大大学分离出了一种能降解吐温80的新型海洋芽孢杆菌菌株N10。基于脂肪酸分析和16S rRNA基因测序，对这种形成芽孢的细菌的分类地位进行了研究。计算机数据库比较分析表明，该细菌是枯草芽孢杆菌菌株。本研究成功利用编码小酸溶性蛋白γ型（SASP-B）的基因sspE作为区分枯草芽孢杆菌两个亚种W23和168的工具。基于16S rRNA序列比对和SASP-B相关性分析，已证明新型海洋枯草芽孢杆菌菌株N10与枯草芽孢杆菌参考菌株W23的亲缘关系比与168更近。菌株N10已保藏于芽孢杆菌遗传保藏中心（BGSC），保藏编号为3A17。
