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具有工业重要性的枯草芽孢杆菌密切相关种的明显分化。

Distinct differentiation of closely related species of Bacillus subtilis group with industrial importance.

机构信息

Microbial Resources Division, Institute of Bioresources and Sustainable Development, Takyelpat Institutional Area, Imphal-795001, Manipur, India.

出版信息

J Microbiol Methods. 2011 Nov;87(2):161-4. doi: 10.1016/j.mimet.2011.08.011. Epub 2011 Aug 26.

DOI:10.1016/j.mimet.2011.08.011
PMID:21889958
Abstract

PCR amplification of 16S rRNA gene by universal primers followed by restriction fragment length polymorphism analysis using RsaI, CfoI and HinfI endonucleases, distinctly differentiated closely related Bacillus amyloliquefaciens, Bacillus licheniformis and Bacillus pumilus from Bacillus subtilis sensu stricto. This simple, economical, rapid and reliable protocol could be an alternative to misleading phenotype-based grouping of these closely related species.

摘要

采用通用引物对 16S rRNA 基因进行 PCR 扩增，然后使用 RsaI、CfoI 和 HinfI 内切酶进行限制性片段长度多态性分析，可将密切相关的解淀粉芽孢杆菌、地衣芽孢杆菌和短小芽孢杆菌从枯草芽孢杆菌中明显区分开来。这种简单、经济、快速和可靠的方案可以替代基于表型的这些密切相关的物种分组方法，避免误导。

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