Kulik E M, Sandmeier H, Hinni K, Meyer J
Institute of Preventive Dentistry and Oral Microbiology, Dental Center, University of Basel, Hebelstr. 3, 4056 Basel, Switzerland.
FEMS Microbiol Lett. 2001 Mar 15;196(2):129-33. doi: 10.1111/j.1574-6968.2001.tb10553.x.
A PCR assay for the amplification of small subunit ribosomal DNA (SSU rDNA) of Euryarchaea was developed and used to detect archaeal rDNA in 37 (77%) out of 48 pooled subgingival plaque samples from 48 patients suffering from periodontal disease. One major group of cloned periodontal sequences was identical to Methanobrevibacter oralis and a second minor group to Methanobrevibacter smithii. These two groups and a third novel group were found to be more than 98% similar to each other over an 0.65-kb segment of the 16S rRNA gene sequenced. M. oralis was found to be the predominant archaeon in the subgingival dental plaque. Phylogenetic analysis of partial SSU rDNA sequences revealed evidence for a distinct cluster for human and animal Methanobrevibacter sp. within the Methanobacteriaceae family.
开发了一种用于扩增广古菌小亚基核糖体DNA（SSU rDNA）的PCR检测方法，并用于检测48例牙周病患者的48份龈下菌斑混合样本中的37份（77%）中的古菌rDNA。克隆的牙周序列的一个主要组与口腔短柄产甲烷菌相同，第二个次要组与史氏短柄产甲烷菌相同。在测序的16S rRNA基因的0.65 kb片段上，发现这两组与第三个新组彼此之间的相似度超过98%。发现口腔短柄产甲烷菌是龈下牙菌斑中的主要古菌。部分SSU rDNA序列的系统发育分析揭示了在甲烷杆菌科内人和动物短柄产甲烷菌属有一个独特聚类的证据。
