人前胸腺细胞中前脑啡肽原mRNA的表达及脑啡肽的产生与分泌。

Expression of preproenkephalin mRNA and production and secretion of enkephalins by human thymocytes.

作者信息

Kavelaars A, Heijnen C J

机构信息

Department of Pediatric Immunology, Room KC 03.068.0, Wilhelmina Children Hospital, University Medical Center Utrecht, PO BOX 85090, 3508 AB Utrecht, The Netherlands.

出版信息

Ann N Y Acad Sci. 2000;917:778-83. doi: 10.1111/j.1749-6632.2000.tb05443.x.

DOI:10.1111/j.1749-6632.2000.tb05443.x

PMID:11268407

Abstract

Human thymocytes were tested for the capacity to express the preproenkephalin (PPE) gene and for production of the end product metenkephalin (MENK). It is shown here for the first time that the cytokines IL-2, IL-4, IL-6, and TGF-beta are capable of inducing PPE mRNA expression. Moreover, a culture of thymocytes with the cytokines results in intracellular expression of MENK as determined by immunohistochemistry. Thymocytes do not secrete detectable amounts of MENK, however, but only the larger MENK-containing peptides or proteins. Cytokines IL-1 beta and IL-10 increase the expression of PPE mRNA in 50% of the thymuses tested, whereas IFN-gamma does not induce changes in PPE mRNA expression.

摘要

对人类胸腺细胞进行了表达前脑啡肽原（PPE）基因的能力以及终产物甲硫氨酸脑啡肽（MENK）产生情况的测试。本文首次表明，细胞因子白细胞介素-2（IL-2）、白细胞介素-4（IL-4）、白细胞介素-6（IL-6）和转化生长因子-β（TGF-β）能够诱导PPE mRNA表达。此外，通过免疫组织化学测定，胸腺细胞与细胞因子的培养导致MENK在细胞内表达。然而，胸腺细胞并不分泌可检测量的MENK，而是仅分泌含较大MENK的肽或蛋白质。细胞因子白细胞介素-1β（IL-1β）和白细胞介素-10（IL-10）在50%的测试胸腺中增加了PPE mRNA的表达，而γ干扰素（IFN-γ）并未诱导PPE mRNA表达的变化。