Zurawski G, Benedik M, Kamb B J, Abrams J S, Zurawski S M, Lee F D
Science. 1986 May 9;232(4751):772-5. doi: 10.1126/science.2938259.
Antigenic or mitogenic stimulation of T cells induces the secretion of an array of protein hormones that regulate immune responses. Molecular cloning has contributed strongly to our present understanding of the nature of this regulation. A complementary DNA (cDNA) library prepared from a cloned concanavalin A-activated mouse T-helper cell line was screened for abundant and induction-specific cDNA's. One such randomly chosen cDNA was found to encode mouse preproenkephalin messenger RNA (mRNA). Preproenkephalin mRNA represented about 0.4 percent of the mRNA in the activated cell line but was absent in resting cells of this line. Other induced T-helper cell lines have 0.1 to 0.5 percent of their mRNA as preproenkephalin mRNA. Induced T-helper cell culture supernatants have [Met]enkephalin-immunoreactive material. The production by activated T cells of a peptide neurotransmitter identifies a signal that can potentially permit T cells to modulate the nervous system.
T细胞的抗原性或促有丝分裂刺激可诱导一系列调节免疫反应的蛋白质激素的分泌。分子克隆对我们目前对这种调节本质的理解有很大贡献。从克隆的伴刀豆球蛋白A激活的小鼠辅助性T细胞系制备的互补DNA（cDNA）文库，被筛选寻找丰富且具有诱导特异性的cDNA。随机选择的一个这样的cDNA被发现编码小鼠前脑啡肽原信使RNA（mRNA）。前脑啡肽原mRNA在激活的细胞系中占mRNA的约0.4%，但在该细胞系的静息细胞中不存在。其他诱导的辅助性T细胞系有0.1%至0.5%的mRNA为前脑啡肽原mRNA。诱导的辅助性T细胞培养上清液中有[甲硫氨酸]脑啡肽免疫反应性物质。活化的T细胞产生一种肽神经递质，这确定了一种可能使T细胞调节神经系统的信号。
