Yhap E O, Wright C B, Popovic N A, Alix E C
J Appl Physiol. 1975 May;38(5):882-5. doi: 10.1152/jappl.1975.38.5.882.
Storage of red cells in acid-citrate-dextrose (ACD) solution for more than a week has been shown to cause a reduction in red cell 2,3-diphosphoglycerate (2,3-DPG), resulting in a decrease in the half-saturation pressure (P50) of stored blood, thus increasing hemoglobin-oxygen affinity and theoretically decreasing oxygen delivery to the tissues. A method to isolate and perfuse the hindlimb in a dog has been developed and used successfully to compare the difference of oxygen release between banked and fresh ACD blood. Ten dogs were perfused using canine blood collected in standard blood transfusion bags containing ACD and stored at 4 degrees C and fresh blood collected in a similar manner but used immediately. The total oxygen release in the limb during perfusion with fresh blood was significantly greater than during perfusion with stored blood.
已证明，红细胞在酸性枸橼酸盐葡萄糖（ACD）溶液中储存超过一周会导致红细胞2,3-二磷酸甘油酸（2,3-DPG）减少，从而使储存血液的半饱和压力（P50）降低，进而增加血红蛋白与氧的亲和力，理论上会减少向组织的氧输送。一种用于分离并灌注狗后肢的方法已被开发出来，并成功用于比较库存ACD血和新鲜ACD血之间的氧释放差异。使用收集在含有ACD的标准输血袋中并储存在4摄氏度的犬血对10只狗进行灌注，以及使用以类似方式收集但立即使用的新鲜血液进行灌注。在灌注新鲜血液期间肢体中的总氧释放量明显大于灌注储存血液期间的总氧释放量。
