Harken A H, Woods M
Ann Surg. 1976 Feb;183(2):130-5. doi: 10.1097/00000658-197602000-00008.
In an intact animal or patient, any shift in oxyhemoglobin affinity is inevitably associated with concurrent fluctuation in numerous other determinants of oxygen delivery. For this reason, the influence of hemoglobin affinity for oxygen on tissue oxygen consumption has been incompletely evaluated. The purpose of this study was to investigate the influence of oxyhemoglobin affinity as the sole variable of oxygen delivery in an isolated perfused canine hindlimb. A membrane lung system which allowed precise control of blood flow, temperature, arterial oxygen content and arterial pH was established. Twelve isolated canine hindlimbs were alternatively perfused with autologous stored (left-shifted) and fresh (right-shifted) blood in paralled perfusion systems. The 2,3-DPG concentrations, P50 and oxygen consumptions were significantly different in the two paralled perfusion systems. A decreased hemoglobin affinity for oxygen appeared to permit increased oxygen off-loading at the tissue level.
在完整的动物或患者体内，氧合血红蛋白亲和力的任何变化都不可避免地与众多其他氧输送决定因素的同时波动相关。因此，血红蛋白对氧的亲和力对组织氧消耗的影响尚未得到充分评估。本研究的目的是在离体灌注的犬后肢中，研究作为氧输送唯一变量的氧合血红蛋白亲和力的影响。建立了一个能够精确控制血流、温度、动脉血氧含量和动脉pH值的膜肺系统。在并行灌注系统中，用自体储存（左移）和新鲜（右移）血液交替灌注12个离体犬后肢。两个并行灌注系统中的2,3-二磷酸甘油酸浓度、P50和氧消耗量有显著差异。血红蛋白对氧的亲和力降低似乎能使组织水平的氧卸载增加。
