Bunn H F, May M H, Kocholaty W F, Shields C E
J Clin Invest. 1969 Feb;48(2):311-21. doi: 10.1172/JCI105987.
Serial oxygen dissociation curves were performed on blood units preserved in acid-citrate-dextrose (ACD), ACD-adenine, and ACD-adenine-inosine. Dividing blood from a single donor into two or more bags allowed direct comparison between preservatives. During the 1st wk of storage in ACD, a progressive increase in oxygen affinity was observed. Thereafter, little further change was noted. Oxygen affinity increased even more rapidly during initial storage in ACD-adenine. However, with the inclusion of inosine as a preservative, oxygen affinity remained unaltered during the first 2 wk. Increases in oxygen affinity correlated well with falling levels of red cell 2,3-diphosphoglycerate (2,3-DPG) during storage. No significant changes in glutathione, reduced form (GSH), or A3 (A(I)) hemoglobin levels were noted during the first 3 wk of storage. No significant accumulation of ferrihemoglobin was detected. When blood stored 20 days in ACD or ACD-adenine was incubated with inosine for 60 min at 37 degrees C, 2,3-DPG and adenosine triphosphate (ATP) were resynthesized, and oxygen affinity was decreased. The distribution of 2,3-DPG in fresh and stored red cells appeared to influence experimental values for Hill's n, a measure of heme-heme interaction.
对保存在酸性枸橼酸盐葡萄糖（ACD）、ACD-腺嘌呤和ACD-腺嘌呤-肌苷中的血液样本进行了连续的氧解离曲线测定。将来自单一供体的血液分装到两个或更多袋子中，以便直接比较不同的保存剂。在ACD中储存的第1周，观察到氧亲和力逐渐增加。此后，未发现进一步的明显变化。在ACD-腺嘌呤中初始储存期间，氧亲和力增加得更快。然而，加入肌苷作为保存剂后，在最初的2周内氧亲和力保持不变。储存期间氧亲和力的增加与红细胞2,3-二磷酸甘油酸（2,3-DPG）水平的下降密切相关。在储存的前3周内，谷胱甘肽（还原型，GSH）或A3（A（I））血红蛋白水平未发现显著变化。未检测到高铁血红蛋白的显著积累。当在ACD或ACD-腺嘌呤中储存20天的血液在37℃下与肌苷孵育60分钟时，2,3-DPG和三磷酸腺苷（ATP）得以重新合成，氧亲和力降低。新鲜和储存红细胞中2,3-DPG的分布似乎会影响希尔系数n的实验值，希尔系数n是血红素-血红素相互作用的一种衡量指标。
