Lin X Z, Jen C M, Sung M Z, Lin J Y, Chou C K, Luo C H
Department of Internal Medicine, Medical College, National Cheng Kung University, Tainan, Taiwan.
Hepatogastroenterology. 2001 Jan-Feb;48(37):91-4.
BACKGROUND/AIMS: Electrochemical therapy is an alternative to treat hepatoma. We compare this method with the other local injection methods on rat liver.
Five groups of Wister rats (24 in each) were anaesthetized. Electrochemical therapy was set under direct current, 6 volts, electrodes were 0.5 cm apart, 0.5 cm deep into exposed parenchyma for 10 min. Local injection was done with 50 microL of 95% alcohol, 30 microL of 20% acetic acid, 30 microL of 35% hydrochloric acid, and 30 microL of 20% sodium hydroxide via a 27-gauge needle under direct vision into each rat. Rats and their livers were examined postmortem on day 1, 3, 7 and 14.
In electrochemical therapy, the treated area showed coagulation necrosis and without blood cells on day 1; then the margin became blurred. Mononuclear cell infiltration, reperfusion and fibrous band formation were prominent from day 3 through day 14. In local injection groups, the necrosis is quite irregular and unpredictable. The regeneration went under similar process.
To destroy tissue locally, electrochemical therapy is unique for its predictability in destructive area and the recovery process and is as effective as the other injection methods. Therefore, it has great potential for hepatoma treatment.
背景/目的：电化学疗法是治疗肝癌的一种替代方法。我们在大鼠肝脏上将这种方法与其他局部注射方法进行比较。
将五组Wister大鼠（每组24只）麻醉。在直流电6伏的条件下进行电化学治疗，电极间距为0.5厘米，插入暴露的实质组织0.5厘米深，持续10分钟。通过27号针头在直视下向每只大鼠局部注射50微升95%乙醇、30微升20%乙酸、30微升35%盐酸和30微升20%氢氧化钠。在第1、3、7和14天对大鼠及其肝脏进行尸检。
在电化学治疗中，治疗区域在第1天显示凝固性坏死且无血细胞；然后边缘变得模糊。从第3天到第14天，单核细胞浸润、再灌注和纤维带形成明显。在局部注射组中，坏死相当不规则且不可预测。再生过程相似。
在局部破坏组织方面，电化学疗法在破坏区域和恢复过程的可预测性方面具有独特性，并且与其他注射方法一样有效。因此，它在肝癌治疗方面具有巨大潜力。
