Tamai T, Seki T, Imamura M, Nakagawa T, Wakabayashi M, Nishimura A, Yamashiki N, Shiro T, Inoue K, Okamura A, Harada K
The Third Department of Internal Medicine, Kansai Medical University, Moriguchi-shi, Osaka 570-8506, Japan.
Oncol Rep. 2000 Jul-Aug;7(4):719-23.
We developed a percutaneous low-concentration alkali injection therapy (PLAIT) targeting hepatocellular carcinoma (HCC), and compared the necrotic areas in the livers of rats that had received PLAIT [an alkaline solution of sodium hydroxide (NaOH)] with those that had received percutaneous ethanol injection therapy (PEIT) and percutaneous acetic acid injection therapy (PAIT). The necrotic area increased with increasing concentrations of NaOH solution. The survival rate of rats was 100% up to a concentration of 4 N; however, the rate dropped below 80% with concentrations over 5 N. Also, at a concentration of 2 N, the necrotic area increased with increasing quantities from 0.01 ml to 0.1 ml. PLAIT using 0.05 ml of 2 N NaOH was 1.56 times more effective than PEIT using 0.05 ml of 99.5% ethanol, and 63.33% less effective than PAIT using 0.05 ml of 50% acetic acid. However, the survival rate after PAIT was 50%, while that after PLAIT was 100%. Histopathological observation of normal rat livers after injection of 2 N NaOH at a volume of 0.05 ml showed that the tissue necrosis spread radially from the site immediately after injection by PLAIT, but necroses were not found in other organs. We conclude that PLAIT has promise as a new form of local therapy for HCC.
我们研发了一种针对肝细胞癌（HCC）的经皮低浓度碱注射疗法（PLAIT），并比较了接受PLAIT（氢氧化钠（NaOH）碱性溶液）的大鼠肝脏坏死区域与接受经皮乙醇注射疗法（PEIT）和经皮乙酸注射疗法（PAIT）的大鼠肝脏坏死区域。坏死区域随NaOH溶液浓度增加而增大。NaOH溶液浓度达4 N时大鼠存活率为100%；然而，浓度超过5 N时存活率降至80%以下。此外，在2 N浓度下，坏死区域随注射量从0.01 ml增加到0.1 ml而增大。使用0.05 ml 2 N NaOH的PLAIT比使用0.05 ml 99.5%乙醇的PEIT效果高1.56倍，比使用0.05 ml 50%乙酸的PAIT效果低63.33%。然而，PAIT后的存活率为50%，而PLAIT后的存活率为100%。对正常大鼠肝脏注射0.05 ml 2 N NaOH后的组织病理学观察表明，PLAIT注射后组织坏死立即从注射部位呈放射状扩散，但在其他器官未发现坏死。我们得出结论，PLAIT有望成为一种新的HCC局部治疗方法。
